创愈膏对大鼠创面肉芽组织中bFGF含量的实验研究

Experiment Study of Chuangyugao on bFGF Content in Granulation Tissue in Rats

目的:检测大鼠体表溃疡创面肉芽组织中bFGF的含量,探索促进溃疡愈合的作用。方法:以60只SD大鼠为研究对象,随机分为A组创愈膏、B组湿润烧伤膏及C组生理盐水三组,每组20只。制造大鼠皮肤全层切除伤模型,每12 h换药1次,分别于造模后3,7,14,21 d时取创基的肉芽组织,用酶联免疫法检测创面bFGF的含量。结果:造模后第3,14,21天,创愈膏组的肉芽组织中bFGF的表达明显高于湿润烧伤膏组及生理盐水组,两两对比有统计学差异;第7天时创愈膏组及湿润烧伤膏组的肉芽组织中bFGF表达无差异,但较生理盐水组均明显降低,有显著差异。结论:创愈膏有增强碱性成纤维细胞的表达作用,从而促进肉芽组织的生长,提高创面的愈合率。

Objective ： To detect the content of bFGF in rat skin ulcer wound granulation tissue and to explore the mechanism of Chuangyugao promoting ulcer healing. Methods ：60 SD rats were randomly divided into Group A（ Chuangyugao）, group B（MEBO） and group C （ normal saline group）, n = 20. The full - thickness excision injury models of those rats were made, changing the dressing every 12 hours. The granulation tissue of wounds was taken out after modeling for 3,7,14,21d, detecting the content of bFGF in wounds with ELISA assay. Results：The expression of bFGF in the granulation tissue in Chuangyugao group was much higher than that of other two groups. There was significant difference through pair wise comparison. And on the 7th day, there was no difference between Chuangyugao group and MEBO group in the expression of bFGF in the granulation tissue, however, compared with the normal saline group, it was significantly lower and there were significant differences. Conclusion： Chuangyugao through the promotion of basic fibroblast proliferation and the growth of granulation tissue can shorten the healing time and improve the rate of wound healing.