不同产地金线莲组培快繁试验

Study on Tissue Culture and Rapid Propagation of Anoectochilus Roxburghii in Different Origins

以不同产地金线莲幼嫩茎段为外植体诱导丛生芽,通过不同培养基配方的筛选,建立不同产地金线莲组培快繁体系。结果表明,不同产地金线莲最佳初代培养基分别是,福建永安产地为MS+2.0 mg/L 6-BA+0.2 mg/L NAA+1.0 mg/L KT,台湾南投产地为MS+3.0 mg/L 6-BA+0.2 mg/L NAA+1.0 mg/L KT,广西玉林产地为MS+4.0 mg/L 6-BA+0.2 mg/L NAA+1.0 mg/L KT;最佳继代培养基分别是,福建永安产地为MS+2.0 mg/L 6-BA+0.2 mg/L NAA,台湾南投产地为MS+3.0 mg/L 6-BA+0.3 mg/L NAA,广西玉林产地为MS+2.0 mg/L 6-BA+0.2 mg/L NAA;在壮苗培养阶段,使用外源添加物蛋白胨后,不同产地金线莲外植体苗的生长状况在不同程度上得到了改善,最佳生根培养基分别是,福建永安产地为1/2MS+1.5 mg/L NAA+0.5 mg/L IBA+2.0 mg/L ABT1,台湾南投产地为1/2MS+0.5 mg/L NAA+0.5 mg/L IBA,广西玉林产地为1/2MS+0.5 mg/L NAA+1.0 mg/L IBA+1.0 mg/L ABT1,同时添加1.5 g/L Ac;炼苗移栽基质V(蛭石)∶V(泥炭土)=1∶1,移栽生根率均达90%以上。

To obtain the system of tissue culture and rapid propagation of Anoectochilus roxburghii from different origins,the tender stem segments of A.roxburghii from different origins were taken as explant,and screened opti-mum culture medium formula to induce shoot clusters.The results showed that：the optimum initial mediums of A. roxburghii in Fujian,Taiwan and Guangxi was MS＋2.0 mg/L 6 -BA＋0.2 mg/L NAA＋1.0 mg/L KT,MS ＋3.0 mg/L 6-BA＋0.2 mg/L NAA＋1.0 mg/L KT,and MS＋4.0 mg/L 6 -BA＋0.2 mg/L NAA＋1.0 mg/L KT,respectively. In addition,the optimum subculture medium of A. roxburghii in Fujian,Taiwan and Guangxi was MS＋2.0 mg/L 6-BA＋0.2 mg/L NAA for,MS ＋3.0 mg/L 6 -BA＋0.3 mg/L NAA for,and MS ＋2.0 mg/L 6-BA＋0.2 mg/L NAA,respectively. In the stage of seedling cultivation,all plants from different produ-cing areas could be improved growth in different degrees after applying exogenous peptone. Additional,the opti-mum rooting medium A. roxburghii in Fujian,Taiwan and Guangxi was 1/2MS ＋1.5 mg/L IBA ＋0.5 mg/L NAA＋2.0 mg/L ABT,1/2MS ＋0.5 mg/L IBA ＋0.5 mg/L NAA,and 1/2MS ＋0.5 mg/L IBA ＋1.0 mg/L NAA ＋1.0 mg/L ABT1 ＋1.5 g/L Ac.Meanwhile,plantlets from test tube grew well on the growth medium ofvermiculitethe and peat soil mixed at the rate of 1∶1,the rooting rate of the A.roxburghii reached 90%.