牛血清白蛋白荧光猝灭测定药物中的头孢孟多酯

Determination of Cefamandole in Drug by Bovine Serum Albumin Fluorescence Quenching Method

应用牛血清白蛋白（BSA）荧光猝灭法建立了一种测定药物中头孢孟多酯（CEF）含量的新方法。牛血清白蛋白具有很强的内源荧光性,而头孢孟多酯溶液本身不产生荧光。当头孢孟多酯与BSA结合后,会导致其荧光强度下降。牛血清蛋白在λex=340 nm处的荧光猝灭程度与头孢孟多酯的量在一定浓度范围内呈良好的线性关系,据此建立测定药品中头孢孟多酯含量的新方法。该结合物的最大发射波长为λmax=340 nm,与头孢孟多酯物质的量浓度在2.18×10^-6~2.62×10^-5 mol·L-1范围内线性关系良好。其线性回归方程为ΔF=2.42×10^7CCEF-35.155,相关系数r=0.996 9,检出限为8.582 58×10-7 mol·L-1,RSD为0.16%,加标回收率为94.67%~98.43%。本方法操作简便、快速,用于实际样本的测定,结果满意。

A new method to detect the cefamandole in drug by fluorescence quenching of bovine serum albumin（BSA）was investigated. The BSA had a strong intrinsic fluorescence because of tryptophan and tyrosine,and cefamandole（CEF） solution itself didn′t produce fluorescence,but when cefamandole combined with the BSA,the fluorescence intensity of BSA was decreased.Based on in a certain range, the fluorescence quenching of BSA in the λex = 340 nm having a good linear relationship with the concentration of cefamandole, a new method to detect the cefamandole in drug was established. The conjugate of BSA and cefamandole had a max fluorescence value at 340 nm which was found the decreased intensity of fluorescence at 340 nm was proportion to cefamandole in the range of 2.18×10^- 6~2.62×10^- 5mol·L- 1. The linear regression equation was ΔF=2.42×10^7CCEF- 35.155 with a correlation coefficient of 0.996 9. The detection limit was 1.029 91×10- 6mol·L- 1,the relative standard deviation was 0.16% and the average recovery of sample was 94.67%~98.43%.The method was simple and rapid,and could be applied to detect the trace cefamandole in drug with satisfactory results.