水杨酸在不同血浆中的蛋白结合率测定

Determination of protein binding rate of salicylic acid in plasma derived from different species via high-per-formance liquid chromatography

目的研究水杨酸与大鼠、比格犬和人的血浆蛋白结合率。方法采用平衡透析法,以高效液相色谱法对透析内液与外液中水杨酸的含量进行测定,计算水杨酸与大鼠、比格犬和人的血浆蛋白结合率。结果水杨酸低、中、高(0.6,1,3μg/ml)3个浓度的大鼠血浆蛋白结合率分别为(91.1±1.4)%,(90.2±2.5)%,(90.7±3.1)%;比格犬血浆蛋白结合率分别为(82.5±2.1)%,(81.3±1.8)%,(83.1±2.3)%;人血浆蛋白结合率分别为(96.5±2.7)%,(97.2±3.0)%,(96.4±1.6)%。结论水杨酸与血浆蛋白具有高强度的结合,其中与人血浆中的蛋白结合较强,且人>大鼠>比格犬。

Objective To investigate the protein binding ratio of salicylic acid in the plasma derived from rats, Beagle dogs and human. Methods Equilibrium dialysis was performed to determine the plasma protein binding rates in plasma derived from different species. High-performance liquid chromatography （HPLC） was employed to determine the concentration of salicylic acid inside or outside of the dialysis membrane, which allowed the calculation of protein binding rates. Results The plasma protein binding rates of salicylic acid at low, middle and high concentrations in different species were （91.1±1.4）%, （90.2±2.5）% and （90.7±3.1）% in rats, （82.5±2.1）%, （81.3±1.8）% and （83.1±2.3）%in Beagle dogs, and （96.5±2.7）%, （97.2±3.0）% and （96.4±1.6）% in humans, respectively. Conclusion The protein binding rates of salicylic acid are highest in human, followed by rats and Beagle dogs.