摘要
为了观察BAPTA-AM脂质体(BAPTA-AM liposome,BA-L)抗大鼠缺血再灌注引起的急性肾损伤(Acute kidney injury,AKI)作用,采取夹闭双侧肾动脉45min后再灌注一定时间致大鼠AKI模型,BA-L治疗组于再灌注开始时立即尾静脉注射BA-L.再灌注24h,检测各组大鼠血清Cr,BUN,Cys C,LDH水平与肾组织匀浆MDA含量和SOD活性,HE染色观察肾组织病理学变化.给予BA-L治疗后AKI大鼠血清Cr,BUN,Cys C,LDH及肾组织匀浆MDA含量显著降低,SOD活性增强,肾功能明显改善,HE染色显示BA-L明显减轻肾小管损伤.结果表明BA-L能显著减轻缺血再灌注引起的大鼠急性肾损伤.
To explore the protective effect of BAPTA-AM liposome （BA-L） on ischemia-reperfusion induced acute kidney injury （AKI） in rats,AKI model was established by clamping the bilateral renal arteries with microvascular clamps for 45 minutes.BA-L at different doses was administered intravenously before reperfusion.After 24 hours of reperfusion,the levels of serum Cr,BUN,Cys C,as well as the activities of LDH were detected to evaluate renal function.Kidney samples were taken for the determination of renal MDA level,SOD activity,and histological examination.Serum Cr,BUN,Cys C levels,as well as activities of LDH were elevated in the model group compared with the control group.In accompany with a significant decrease in SOD activity,the MDA level was significantly increased in the kidney tissues homogenate in the AKI model group.BA-L could significantly block the elevation of serum levels of Cr,BUN,Cys C and LDH.BA-L could significantly block the increase of MDA levels and the depletion of SOD activity in the kidney tissues homogenate in the AKI model group.These beneficial changes in the biochemical parameters were also associated with parallel changes in histopathological appearance.The results above demonstrate that BA-L can exert an admirable protection against ischemia-reperfusion induced acute kidney injury in rats.
出处
《浙江工业大学学报》
CAS
2014年第5期491-495,共5页
Journal of Zhejiang University of Technology
