木瓜蛋白酶水解酪蛋白制备降血压肽的工艺优化

Optimization of preparation process of casein ACE inhibitory peptides with papain

目的:以酪蛋白为原料,酶解法制备降血压肽,优化工艺。方法:采用5种不同蛋白酶水解酪蛋白,根据水解产物的血管紧张素转化酶(ACE)体外抑制活性选择蛋白酶。以酶用量、酶解时间、底物浓度3因素作为研究对象,以可溶性蛋白产量、ACE体外抑制活性两参数为评价指标,通过Box-Behnken响应面分析法优化水解工艺。结果:木瓜蛋白酶水解酪蛋白的产物活性最高,因此作为工艺优化用酶。响应面分析法得到的最优工艺条件为:酶用量0.032 g,酶解时间4.53 h,底物浓度0.70%,可溶性蛋白产量1283.71 mg、ACE抑制率为87.23%。结论:Box-Behnken设计可有效优化木瓜蛋白酶水解酪蛋白制备降血压肽工艺,所得工艺稳定可靠。

Objective： Casein was hydrolyzed using protease to produce protein hydrolysate and the preparation conditions was optimized. Methods： Casein was hydrolyzed using five difererent proteases and the protease was choosed by angiotensin-I converting enzyme （ACE） inhibitory activity of hydrolysate. Box-Behnken response surface methodology was used to optimize the preparation process. The independent variables were investigated, including the amount of enzyme （Xl）, hydrolysis time （X2） and concentration of casein （X3）. Yield of solubility protein and ACE-inhibitory activity （IP） were desiged as the dependent variable. Results： Two binomial equations was established to describe the correlation between the variables and the response. The optimum conditions are that the amount of enzyme of 0.032 g, hydrolysis time of 4.53 h and concentration of casein of 0.70%. Under these conditions, yield of solubility protein and ACE-inhibitory activity （IP） were 1283.71 mg and 87.23%, respectively. Conclusion： Box-Behnken was successfully used to optimize the preparation process of the casein hydrolysate with papain. The optimized process is reliable, stable, and available.