假酸浆中总黄酮的提取及稳定性研究

Extraction and stability of the total flavonoids in the Nicandra physaloides

应用超声波微波法和超声波辅助双水相法提取假酸浆中总黄酮,得到总黄酮提取的最佳工艺条件,在最佳条件下对所提取的黄酮进行稳定性研究。结果表明,超声波辅助双水相法提取总黄酮的提取率为3.79%,超声波微波法提取总黄酮的提取率为1.01%。假酸浆黄酮在pH4比较稳定,温度超过40℃时稳定性较差,光照会使黄酮稳定性降低,氧化剂还原剂对黄酮稳定性有一定的影响,葡萄糖对黄酮稳定性影响不明显,蔗糖和Vc对黄酮稳定性影响较大,Ca2+、Cu2+、Fe3+对黄酮稳定性影响较大,Na+、K+、Mg2+对黄酮稳定性影响不大。

The total flavonoids in the Nicandra physaloides are extracted by the use of ultrasonic microwave method and ultrasonic assisted aqueous two-phase method so as to obtain the best condition for the extraction of the total flavonoids, under which the study on the stability of the total flavonoids can be done. The results showed that by the use of ultrasonic assisted aqueous two-phase method, the extraction rate of the total flavonoids is 3.79%. By the use of ultrasonic microwave method, the extraction rate of the total flavonoids is 1.01%. The total flavonoids in the Nicandra physaloides are relatively stable around pH4 while it is not very stable in the temperature exceeding 40 %. The light can bring down the stability of the flavonoids. The oxidant and the reducing agent have a certain impact on its stability. And glucose poses no obvious effect while sucrose and Vc have an significant impact. Ca2＋、Cu2＋、Fe3＋pose an significant impact while Na＋、K＋、Mg2＋ has little effect on the stability of flavonoids.