豆腐中特定腐败菌的生长预测模型研究

The growth kinetics prediction model of specifics spoilage bacteria in tofu

为探讨豆腐中特定腐败菌坚强芽孢杆菌（Bacillus firmus）的生长规律,将坚强芽孢杆菌接种到豆腐表面,在1、5、9、13℃条件下贮藏培养,通过修订的Gompertz方程非线性拟合不同温度下坚强芽孢杆菌的生长曲线;同时,采用平方根方程对温度与坚强芽孢杆菌最大比生长速率和延滞时间线性拟合,并在此基础上建立豆腐中特定腐败菌坚强芽孢杆菌的生长预测模型。通过对所建立的生长预测模型的进行验证,偏差度Bf和准确度Af分别在1.04~1.10和1.06~1.12之间,表明所建模型有效可靠,可为定量评价豆腐特定腐败菌的生长预测提供有效的手段。

The paper studies the growth regularity of the specific spoilage bacteria Bacillus firmus in tofu. the Tofu was inoculated by Bacillus firmus, then stored at 1, 5, 9, 13 ℃. The growth curves of Bacillus firmus was nonlinear fitted by the modified Gompertz equation under different temperatures, then the maximum specific growth rate and delay time of Bacillus firmus in tofu were also linear fitted by Belehradek equation. On the basis, the growth kinetics prediction model of specifics spoilage bacteria Bacillus firmus in tofu was established. Validation of built kinetic model was carried out, and the results show that bias and accuracy factors of model were in 1.04-1.10 and 1.06--1.12, which showed that the model is effective and reliable. The results of research provide an effective method for the quantitative evaluation of the growth prediction of specific spoilage bacteria in tofu.