痰标本流感嗜血杆菌的分离培养及耐药性分析

The Isolated Culture and Analysis on the Antibiotic Resistance of Haemophitus Influenzae in Sputum

目的提高痰培养流感嗜血杆菌的检出率,了解其耐药性,为经验用药提供依据。方法对本院近两年来痰标本分离的流感嗜血杆菌,总结分离培养经验及药敏结果,采用WHONET5.6软件处理数据。结果痰培养共分离流感嗜血杆菌61株,对复方新诺明、四环素、氨苄西林的耐药率较高,分别是50.8%、37.7%和27.9%,对左氧氟沙星等耐药率小于10%。结论微生物室在痰培养中应从控制合格痰标本等环节提高流感嗜血杆菌的检出率。左氧氟沙星等大多数药物耐药率较低,可作为治疗流感嗜血杆菌感染的经验药物,复方新诺明、四环素不适合临床经验用药。

Objective To increase the detection rate of Haemophitus Influenzae in sputum culture and investigate the antibiotic resistance of clinical isolates of haemophilus influenzae for providing basis of the empirical antibiotic use. Method The experience of isolated culture and the antimicrobial susceptibility result was summarized to Haemophitus Influenzae in sputum culture in the last two years. WHONET 5.6 software was used to process data.Results 61 strains haemophilus influenzae were separated from sputum culture, Resistant rate of haemophilus influenzae to TMP/SMZ,tetracycline,ampicillin were higher,they were 50.8%, 39.7% and 27.9%, respectively, while resistant rate of levofloxacin etc was less than 10%. Conclusion Moicrobiology lab should improve the detection rate from controlling the qualified sputum specimen etc in sputum culture.The resistant rate to the majority of antibiotic such as levofloxacin is low,they are suitable to treat infections caused by haemophilus influenzae for experiential therapy, TMP/SMZ, tetracycline are unsuitable.