提取玫瑰基因组DNA几种方法的比较

Comparison of Several Methods in Genomic DNA Extraction from Rosa rugosa

为了找到一种方便、快捷且经济高效的玫瑰叶片基因组DNA提取方法,为玫瑰后期的分子生物学研究奠定良好基础,以不同品种的玫瑰幼嫩叶片为材料,分别利用CTAB法、SDS法、改良CTAB法、试剂盒法4种方法提取玫瑰基因组DNA,经过琼脂糖凝胶电泳、紫外分光光度法对所提DNA的质量和产量进行检测比较。结果表明:4种方法均可从玫瑰叶片中提取到DNA,改良CTAB法作为对常规方法的改进,在保证经济实用的前提下提取到的DNA质量最好,浓度最高;而试剂盒法作为一种快速提取植物基因组DNA的新兴方法,虽然产量略低,费用略高,但操作步骤简单,耗时短,毒害小,高效快捷。各实验室可根据实际情况选择适宜的方法。

In order to find a convenient, fast and cost-effective genomic DNA extraction method and lay a good foundation for the study of Rosa rugosa, its genomic DNA was extracted from young leaves of different varieties of Rosa rugosa with CTAB method, SDS method, modified CTAB method and DNA minprep kit method. The quality and quantity of the extracted DNA was tested and compared by means of agarose gel electrophoresis and UV specterphotometry. The results showed that DNA could be extracted with all the four methods, but as an improved method, modified CTAB method could extract DNA of the best quality and the highest concentration under the premise of being economical and practical. However, DNA minprep kit method was a new method of DNA rapid extraction. This method had disadvantages of lower yield and higher cost, but had advantages of simpler operation, shorter time, lower toxicity, higher efficiency and faster. A suitable method could be chosen according to the actual conditions of the labs.