博尔纳病病毒感染与精神分裂症关系的初步研究

A preliminary study on the relationship between Borna disease virus infection and schizophrenia

目的:探讨精神分裂症患者发病与博尔纳病病毒(BDV)感染的关系,分析被BDV感染的精神分裂症患者临床特征。方法:用荧光定量巢式逆转录聚合酶链反应(FQ-nRT-PCR)方法检测86例精神分裂症患者(病例组)和84例健康体检者(对照组)外周血单个核细胞(PBMCs)中BDVp24和BDVp40基因片段,用β-肌动蛋白(β-actin)作为内参照,并总结阳性患者的临床特征,病例组采用阳性与阴性症状量表(PANSS)评分。结果:病例组外周血标本BDV p24基因片段检出率11.6%(10/86),BDV p40基因片段检出率14.0%(12/86),拷贝数均>102kb/μl。对照组BDV p24、BDV p40基因片段均为阴性(0%,0/84),两组阳性率差异有统计学意义(P均<0.05)。BDV p24和BDV p40基因片段均为阳性的标本测序后,与BDV标准病毒株V和马源的BDV病毒株H1766序列比较同源性分别为96.35%和98.85%。在4个位点出现基因突变(nt1649 T→C、nt1656 G→A、nt1670 C→T和nt1676 C→T)。该目的基因片段与马源的BDV病毒株亲缘关系最近。BDV阳性患者精神症状主要以幻觉、妄想为主。结论:精神分裂症发病与BDV感染有一定的相关性,BDV阳性患者以阳性精神症状为主要临床特征。

Objective：To explore the relationship between the patients with schizophrenia and Borna dis-ease virus（BDV）infection as well as analyze the clinical features of patients infected by BDV. Method：The p24 and p40 genes fragment of BDV in patients with 86 schizophrenic patients（case group）and 84 healthy con-trols（control group）were examined in peripheral blood mononuclear cells（PBMCs）by nested with fluorescence quantitative nested real-time polymerase chain reaction（FQ-nRT-PCR）. β-actin in specimens were detected by PCR as internal reference. The clinical characteristics of BDVp24 or BDVp40 positive patients were summa-rized. Positive and negative syndrome scale（PANSS）was used to evaluate case group. Results：The positive rate（10 / 86,11. 6% ）and（12 / 86,14. 0% ）of BDV p24 and p40 in PBMCs in case group were significantly higher than that in control group（0 / 84,0% ）（all P 〈 0. 05）,and the number of copies of BDV p24 and p40 in PBMCs were more than 102 kb/ μl. The homology of the gene sequence of positive product of PBMCs were 96. 35% and 98. 85% ,which had 4 situs of consistent silent mutation（nt1649 T→C,nt1656 G→A,nt1670 C→T, nt1676 C→T）,compared with the sequence of the standard strain V and the virus strain H1766 of BDV from horse supplied by GenBank. The homology of the gene sequence of positive product was genetically relative to the virus strain BDV from horse. The clinical features in BDV positive patients with schizophrenia were mainly hallucination and delusion. Conclusion：BDV infection and schizophrenia may have certain relations. The clinical features in BDV positive patients with schizophrenia are mainly positive symptoms.