一氧化氮、过氧化氢参与光诱导绿豆下胚轴花青素合成

Effect of Nitric Oxide, Hydrogen Peroxide on Light-induced Anthocyanin Synthesis in Mung Bean Hypocotyl

为研究光诱导花青素合成的信号转导过程,以绿豆下胚轴为材料,借助生理学方法、组织学方法和分光光度法,测量了光对花青素合成的效应、光诱导花青素合成的部位及一氧化氮(NO)、过氧化氢(H2O2)在光诱导花青素合成中的作用。结果显示,光诱导了花青素合成,花青素含量随光照时间延长而增加;花青素的合成部位为表皮下薄壁细胞;≥10 mmol/LNO合酶抑制剂L-NAME、≥300μmol/L硝酸还原酶抑制剂Na2WO4、≥300μmol/LNO清除剂血红蛋白(Hb)、≥3μmol/LH2O2产生酶NADPH氧化酶抑制剂DPI、≥10 mmol/LH2O2清除剂抗坏血酸(Vc)、≥200 units/mLH2O2清除酶过氧化氢酶(CAT)均显著抑制光诱导花青素合成,表明NO和H2O2作为中间信号分子参与光诱导的花青素合成。

To study the light signaling process in anthocyanin synthesis, by means of physiological, histological method and spectrophotometry, the effete of light on anthocyanin synthesis, location of anthocyanin synthesis and the action of NO and H2O2 in light-induced anthocyanin synthesis were measured in mung bean hypocotyl. The results show that light induced anthocyanin synthesis, and the effect was time-dependent. The location of anthocyanin synthesis was parenchyma cells under epidermis in hypocotyl of mung bean. Further, lightinduced anthocyanin synthesis was largely prevented by 〉110 mmol/L NO synthase inhibitor L-NAME, ≥300 μmol/L nitrate reductase inhibitor Na2WO4, ≥300 μmot/L NO scavenger hemoglobin （Hb）, ≥3 μmol/L DPI, an inhibitor of the H2O2-generating enzyme NADPH oxidase, 210 mmol/L Vc, an important reducing substrate for H202 removal, or≥200 units/mL H2O2 scavenging enzyme CAT, indicating that NO and H2O2, as intermediate signal molecule, mediated light-induced anthocyanin synthesis.