基于丽江新团黑谷的稻瘟病菌致病性分化

Pathogenicity differentiation of rice blast pathogen(Magnaporthe grisea)based on Lijiangxintuanheigu

为明确普感品种丽江新团黑谷中稻瘟病菌致病性的分化情况,将分离的100个单孢菌株分别接种到7个中国传统鉴别品种和30个抗瘟单基因品系上,根据接种品种的抗感反应确定稻瘟病菌的优势小种类型、生理小种组成、致病力及其所含无毒基因等信息,并与从同一地区其他寄主品种分离到的稻瘟病菌种群的致病性比较。结果表明:从丽江新团黑谷上分离的稻瘟病菌与从整个区域不同品种上分离的病菌致病性分化情况相似,其中从丽江新团黑谷分离的菌株各种群组成为小种ZA占85.00%、ZB占13.00%、ZC占2.00%,优势小种为ZA1、ZA5、ZB13,菌株平均毒性频率为83.33%;从其他寄主品种分离的菌株各种群组成为ZA占53.85%、ZB占38.46%、ZC占5.77%、ZD占1.92%;优势小种为ZA1、ZB15、ZB13,菌株平均毒性频率为78.01%。

To clarify the pathogenicity differentiation of Magnaporthe oryzae in the generally susceptible variety Lijiangxintuanheigu（LTH）,7Chinese traditional differential varieties and 30anti-blast monogenic lines were inoculated respectively with 100 single spore strains isolated from Lijiangxintuanheigu.According to the inoculation results,the composition of physiology races,the types of dominant races,virulence and avirulence genes of Magnaporthe oryzae were found.The above results were compared with the pathogenicity of Magnaporthe oryzae from other cultivars in the one region,and it showed that the pathogenicity differentiation of Magnaporthe oryzae from Lijiangxintuanheigu was similar to those isolated from other varieties in the area.The population composition of Magnaporthe oryzae from Lijiangxintuanheigu was ZA（85.00%）,ZB（13.00%）,ZC（2.00%）,dominant races were ZA1,ZA5,ZB13;the average virulence was 83.33%.And the population composition of Magnaporthe oryzae from other cultivars were ZA（53.85%）,ZB（38.46%）,ZC（5.77%）,ZD（1.92%）;dominant races were ZA1,ZB15,ZB13;the average virulence was 78.01%.So the study of monitoring physiology races of Magnaporthe oryzae was of great reference value in the identification of resistance genes of rice cultivar and acquisition of rice blast samples.