摘要
目的观察P53凋亡刺激蛋白(ASPP家族的抑制因子iASPP)在压力诱导的视网膜神经节细胞(RGC-5)凋亡中的表达及意义。方法将视网膜神经节细胞(RGC-5细胞)分别在60mmHg和80mmHg压力条件下培养48小时,MTT方法检测细胞活力,细胞染色检测凋亡,RT-PCR,WESTERN-BLOT检测P53和iASPP基因的变化。结果随着压力的增加RGC-5细胞活力下降,细胞凋亡逐渐增加,P53基因表达增加,iASPP基因的表达下降。结论在压力诱导的RGC-5细胞凋亡中iASPP表达下降可能使P53活性增加,促进RGC-5细胞凋亡的发生,上调iASPP表达可能具有神经保护作用。
Objective This study was to explore the expression of iASPP on pressure-induced apoptosis of cultured retinal ganglion cells (RGC-5 cells).Methods The RGC-5 cells were cultured under the pressure of 60mmHg and 80mmHg for 48 hours.Cell viability were determined by MTT assays.Cells apoptosis was determined by Hoe33342. The expression of iASPP and P53 was determined by using semi-quantitative RT-PCR and Western blot respective. Results MTT assay showed that the RGC-5 cells viability was decreased in a pressure-dependent manner.As the pressure was up,the apoptosis of RGC-5 cells was increased,the expression of P53 was elevated and the expression of iASPP was decreased.Conclusion The reduced expression of iASPP gene may increase the activity of p53 and promote the apoptosis of RGC-5 cells under pressure.Enhancing expression of iASPP may play a role as a neuroprotect.
出处
《中国实验诊断学》
2014年第10期1589-1591,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅资助项目(20090460)