卡介苗在关节炎模型小鼠诱导中的作用

Roles of BCG in establishing collagen-induced arthritis mice

目的:探讨在牛Ⅱ型胶原诱导性关节炎模型(Collagen-induced arthritis,CIA)小鼠的诱导过程中,弗氏完全佐剂(FCA)中的卡介苗(BCG)浓度和CIA小鼠关节炎严重程度的关系。方法:将牛Ⅱ型胶原蛋白溶液与含不同BCG的FCA等体积混合乳化,在小鼠尾根部注射进行免疫。免疫后观察小鼠的相关指标包括体重、发病率、血清TNF-α、关节炎指数及小鼠关节和脾脏的病理变化。结果:与1 mg/ml卡介苗组相比,4 mg/ml卡介苗组诱导的CIA小鼠发病早,关节炎指数、关节和脾脏病理评分升高及小鼠体重下降更明显,血清TGF-α明显升高,差异有统计学意义。结论:在进行CIA小鼠诱导时,BCG浓度和CIA小鼠关节炎严重程度呈正相关,实验中可以根据对动物的要求,选择含不同BCG的佐剂来诱导CIA小鼠。

Objective：To determine the correlation of BCG concentration in Freund complete adjuvant（FCA） and severity of arthritis during arthritis establishment in DBA1 /j male mice induced by bovine type Ⅱ collagen. Methods： CIA was induced by the immunization of DBA1 /j mice with bovine type Ⅱ collagen and BCG of various concentrations dissolved in FCA. To ascertain the effects administering the collagen booster,CIA-related features（ including body weight,clinical scoring of arthritis）,TNF-α in serum and the histopathological changes in the spleen and the joints regions were measured. Results： 4 mg /ml BCG induced more serious arthritis than 1 mg /ml in DBA1 /j mice after collagen exposure. Conclusion： There is a positive correlation of arthritis severity with BCG. Which indicates that selection of adjuvant with suitable BCG concentration could determine pathological outcome in CIA mouse model.