IL-17在类风湿关节炎患者外周血表达的临床意义

Clinical significance of IL-17 in peripheral blood of patients with rheumatoid arthritis

目的:探讨IL-17在类风湿关节炎(RA)发病中的作用及意义。方法:采用逆转录-聚合酶链反应(RT-PCR)法检测66例RA患者(活动期34例、缓解期32例)和44例健康对照组外周血单个核细胞(PBMC)中IL-17 mRNA的表达水平,采用酶联免疫吸附试验(ELISA)检测各组血清中IL-17的浓度,用免疫比浊法检测各组血清RF的浓度,分析血清IL-17与RF的相关性。结果:RA患者PBMC中IL-17 mRNA的表达显著高于健康对照组(P<0.05),但活动期与缓解期无明显差异(P>0.05);RA活动期患者血清IL-17水平显著高于RA缓解期患者和健康对照组(P<0.05);且RA患者血清IL-17水平与RF呈正相关。结论:IL-17和RF在RA的发病和病理过程中起重要作用,IL-17可能与RA活动性有关。

Objective：To explore the role and significance of IL-17 on the pathogenesis of rheumatoid arthritis（RA）. Methods：The levels of IL-17 mRNA in peripheral blood mononuclear cell（ PBMC） of 66 RA patients（32 active,34 inactive） and 44 healthy controls were detected by reverse transcription-polymerase chain reaction（RT-PCR）. The levels of serum IL-17 were detected by enzyme-linked immunosorbent assay（ELISA）. The levels of RF were detected by immunoturbidimetric assay. The correlations of serum IL-17 and RF were analyzed. Results： In RA patients the levels of IL-17 mRNA in PBMC were significantly higher than those in healthy controls（P〈0. 05）,but there was no difference between the active and inactive RA（P〉0. 05）. The serum IL-17 levels in active RA patients were significantly higher than those in inactive RA patients and healthy controls（P〈0. 05）. The levels of serum IL-17 were positively correlated with RF in RA patients. Conclusion： IL-17 and RF play an important role in the pathogenesis and pathology process of RA,and IL-17 may be associated with RA activity.