UPLC-MS/MS法测定液态乳中舒巴坦残留

Determination of sulbactam residues in milk by UPLC-MS/MS method

建立了牛乳中舒巴坦的超高效液相色谱-串联质谱（UPLC-MS/MS）检测法。牛乳中的目标物经体积分数为0.2%乙酸-水溶液提取后,经HLB固相萃取柱净化、浓缩,再用超高效液相色谱-串联质谱仪进行测定,外标法定量。方法的回收率为83.3%～116.3%,且相对标准偏差为1.9%～8.6%。该方法快速、准确,回收率高,可用于牛乳中舒巴坦的快速检测。

The paper establishes a method to determine sulbactam content in milk using UPLC-MS/MS. Acetic acid solution was added to milk samples to extract target ingredient, and then concentrated by HLB SPE procedureapp：ds：successively. Sulbactam content was measured by UPLC-MS/MS. The recoveries of sulbactam are 83.3%～116.3%, and the relative standard deviations are 1.9%～8.6%, shows that the method is meet the demand to determine sulbactam content in milk.