尾悬吊大鼠小脑间位核促离子型谷氨酸受体表达增加

Increased iGluRs expression of interpositus nuclear in rat under tail-suspension

外周信息主要通过神经末梢释放谷氨酸激活促离子型谷氨酸受体(ionotropic glutamate receptor,iGluRs),调节间位核神经元的兴奋性。研究旨在探讨模拟失重对大鼠小脑间位核iGluRs表达的影响。采用大鼠尾部悬吊法建立模拟失重动物模型,按体重配对原则随机将大鼠分为尾部悬吊14 d组和正常同步对照组。采用免疫组织化学ABC染色法观测大鼠小脑间位核iGluRs(NMDAR-1、AMPAR-2和KAR-2)免疫阳性神经元数量的变化。结果表明正常对照组大鼠小脑间位核内有iGluRs免疫阳性神经元存在。大鼠吊尾14 d后,大鼠小脑间位核iGluRs免疫阳性细胞数明显增多(P<0.05)。结论模拟失重可使大鼠小脑间位核神经元网络的兴奋性增高。这一变化可能与模拟失重引起的调控肌肉运动的中枢神经系统的适应性变化有关。

To study the effects of 14 days of simulated weightlessness on ionotropic glutamate receptors （iGluRs） immunoreactivity in the Interpositus nuclear neurons of rat, immunohistochemistry ABC staining method was used.Results showed that the numbers of NMDAR-1, AMPAR-2 and KAR-2 immunoreactive neurons in the interposed cerebellar nucleus were all obviously increased after 14 days of tail-suspension （ P〈0.05）.The findings suggested that weightlessness induces an obvious increase in the activation of neuro-nal circuit in the interposed cerebellar nucleus.