东风桔对大鼠慢性支气管炎的治疗作用及其机制研究

Therapeutic effect of Atalantia buxifolia on chronic bronchitis and its mechanism

目的研究东风桔乙醇提取物及其萃取部位对慢性支气管炎大鼠的治疗作用,并初步探讨其作用机制。方法以二氧化硫熏制法制备大鼠慢性支气管炎模型,东风桔乙醇提取物及其氯仿、正丁醇萃取部位分别ig给药,分析大鼠支气管肺组织病理变化,并用ELISA法测定肺组织匀浆中白细胞介素(IL)-10、IL-4、肿瘤坏死因子-α(TNF-α)水平变化。结果东风桔乙醇提取物和正丁醇萃取部位可以改善慢性支气管炎大鼠的倦怠无力、进食减少和体质量不增等身体状况;使大鼠支气管黏膜炎症细胞浸润程度、黏膜上皮细胞脱落减轻,支气管腔内分泌物减少,可抑制支气管黏膜上皮细胞增生,使支气管内径增大,通气量增大,而且东风桔乙醇提取物低剂量组和正丁醇低剂量组可显著降低慢性支气管炎大鼠肺组织匀浆中升高的TNF-α水平,同时使下降的IL-10、IL-4水平升高,与模型组比较有统计学意义(P<0.05)。结论东风桔乙醇提取物和正丁醇萃取部位对慢性支气管炎的治疗效果较好,其对慢性支气管炎的治疗作用可能与其平衡细胞因子产生有关。

Objective To evaluate the effect of the ethanol extract and active parts from the roots of dtalantia buxifolia on chronic bronchitis （CB） and their mechanism. Methods The rat model of CB was established with smoking method of sulfur dioxide, ethanol extract and active parts from the roots of A. buxifolia were ig administrated, the pathological changes of lung tissue in rats were analyzed, and the level changes of intefleukin-10 （IL-10）, interleakin-4 （IL-4）, and tumor necrosis factor -ct （TNF-c0 were determined by ELISA method. Results The administration of ethanol extract and n-butanol extract could improve the tiredness and weakness of CB rats, reduced food intake, and body weight without increasing status, decrease the degree of infiltration, epithelial cell shedding, bronchial secretion, inhibit the proliferation of bronchial epithelial cells of the rat bronchial inflammatory cells, and make the bronchial diameter increases and ventilation. The low dose of the ethanol extract and n-butanol extract could significantly inhibit the increase of TNF-ct, IL-10, and IL-4 in the lung tissue of rats with CB in the homogenate, compared with the model group with the statistical significance （P 〈 0.05）, but compared with the positive group without significant difference （P 〉 0.05）. Conclusion The alcoholic extract and n-butanol extract in the roots ofA. buxifolia have the better treatment effect on CB, which may be related to the balance of cytokine production related.