超临界CO_2萃取二级分离火麻仁油中生育酚及主要甘油三酯组成分析

Compositions of tocopherol and triglyceride of hemp seed oil by supercritical CO_2 extraction and two-step separation

以脱壳火麻仁为原料,采用超临界CO2萃取二级分离火麻仁油,通过控制分离参数,分离釜Ⅰ及分离釜Ⅱ分别收集火麻仁油,应用超高效液相色谱-高分辨质谱联用及国标方法分别分析比较其甘油三酯及生育酚组成。结果表明:分离釜Ⅰ及分离釜Ⅱ得到的火麻仁油甘油三酯及生育酚组成和含量存在一定差异,分离釜Ⅰ火麻仁油的甘油三酯组成主要包括三亚油酸甘油三酯、二亚油酸亚麻酸甘油三酯及油酸二亚油酸甘油三酯,含量分别为32.1%、29.8%及19.6%;分离釜Ⅱ火麻仁油的甘油三酯组成主要包括二亚油酸亚麻酸甘油三酯、三亚油酸甘油三酯及油酸二亚油酸甘油三酯,含量分别为33.9%、27.6%及26.4%。火麻仁油生育酚以γ-生育酚为主,其次为α-生育酚,δ-生育酚最少,分离釜Ⅱ中火麻仁油生育酚含量高于分离釜Ⅰ中火麻仁油生育酚含量。采用超临界CO2萃取二级分离火麻仁油,可以初步实现甘油三酯及生育酚组分的选择性分离。

Using shelled hemp seed as raw material, hemp seed oil was extracted by supercritical CO2 extraction and two -step separation and was collected separately from separation kettle Ⅰ and separation kettle Ⅱthrough controlling the separation parameters. Then the compositions of triglyceride and tocopherol of the hemp seed oil obtained were analyzed by ultra high performance liquid chromatography - mass spectrometry method and national standard method respectively. The results showed that there were some differences in compositions of triglyceride and tocopherol of hemp seed oils collected from separation kettle Ⅰ and separation kettle Ⅱ. Hemp seed oil gathered from separation kettle Ⅰ basically consisted of trilinolein（32.1% ）, dilinolenic acidlinoleic acid triglyceride（29.8% ）and oleic aciddilinoleic acid triglyceride （ 19.6% ） ; while hemp seed oil gathered from separation kettle Ⅱ basically consisted of dilinoleic acid - linolenic acid triglyceride （33.9%）, trilinolein （ 27.6% ） and oleic acid - dilinoleic acid triglyceride （26.4%）. Tocopherol composition of hemp seed oil was main γ- tocopherol, followed by α - tocopherol, δ - tocopherol was the least. Tocopherol content of hemp seed oil collected from separation kettle Ⅱ was more than that collected from separation kettle Ⅰ . By supercritical CO2 extraction cou-pled with two- step separation, the selective separations of triglyceride and tocopherol of hemp seed oil could be initially achieved .