板蓝根不同提取部位抗炎镇痛活性比较研究

Comparative studies on anti-inflammatory and analgesic activities of different fractions from Isatidis Radix

目的比较不同板蓝根提取部位的抗炎、镇痛活性。方法采用脂多糖(LPS)刺激小鼠巨噬细胞RAW264.7模型,检测板蓝根不同提取部位对肿瘤坏死因子-α(TNF-α)及前列腺素E2(PGE2)的影响,考察其抗炎活性;采用醋酸扭体法及热水缩尾法2种动物模型考察板蓝根提取物的镇痛活性。结果板蓝根水洗及30%、50%、70%乙醇提取4个部位均具有一定的抗炎、镇痛活性,其中抗炎的有效部位集中在50%、70%乙醇提取部位;镇痛的有效部位集中在水洗部位以及70%乙醇提取部位。结论板蓝根的不同提取部位均具有抗炎、镇痛活性,板蓝根70%乙醇提取部位在抗炎、镇痛活性上均显示出较强的作用,其机制可能与抑制炎性因子PGE2及TNF-α释放有关。

Objective To observe the anti-inflammatory and analgesic activities of the different fractions from Isatidis Radix. Methods The anti-inflammatory effects were observed by a in vitro model of LPS-stimulated RAW264.7 macrophages, and the analgesic effects were evaluated by the writhing test and the tail-immersion test in mice. Results The water extract, 30%, 50%, and 70% ethanol extracts of Isatidis Radix all had anti-inflammatory and analgesic activities. The 50% and 70% ethanol extracts were the main anti-inflammatory effective fractions. The water extract and 70% ethanol extract were the main analgesic effective fractions. Conclusion Different fractions of Isatidis Radix all have anti-inflammatory and analgesic activities, and the 70% ethanol extract has stronger activities. The mechanism of the anti-inflammatory and analgesic activities of the 70% ethanol extract may be related to the inhibition of the release of TNF-α and PGE2 from macrophages.