谷氨酰胺对点带石斑鱼免疫细胞免疫力的影响

Effects of Glutamine on Immune Responses of Immunocytes in Grouper Epinephelus malabaricus

将从平均体质量400g的点带石斑鱼中肾分离出的白细胞培养在添加0、0.5、1、2mmol/L谷氨酰胺的培养基中2、6、12、24h后,测定白细胞的吞噬和呼吸爆发活力,结果发现,添加谷氨酰胺后白细胞的吞噬和呼吸爆发活力显著提高,随着培养时间的延长,吞噬和呼吸爆发活力进一步提高,最高值均在培养12h后的1mmol/L组。在培养12、24h后测定了白细胞的增殖能力,发现培养12h后,添加谷氨酰胺的各组增殖效果明显优于未添加谷氨酰胺组(对照组)(P<0.05),但添加谷氨酰胺的各组之间差异不显著(P>0.05),最高值在1mmol/L组;培养48h后,除2mmol/L组外,其他处理组的增殖效果均下降。白细胞对迟钝爱德华氏菌杀菌率变化趋势与吞噬活力相符,1、2mmol/L组杀菌率显著高于对照组(P<0.05),而0.5mmol/L组与对照组之间差异不显著(P>0.05)。试验结果表明,谷氨酰胺是石斑鱼有效的免疫增强剂,在培养基中添加1mmol/L谷氨酰胺培养12h效果最佳。

An ivitro experiment was conducted to evaluate the effects of glutamine（0,0.5,1,2mmol/L）on immune responses of kidney leukocytes in grouper（Epinephelus malabaricus）.After the leukocytes were cultured primarily in 2,6,12,24 h,the phagocytosis and respiratory burst activity of leukocytes were determined,respectively.Phagocytosis and respiratory burst activity of leukocytes were significantly enhanced with glutamine supplemented into the culture medium,and increased further with the increasing culture time.The peak was occurred in 1mmol/L glutamine group 12 hafter incubation.The proliferation of leukocytes was determined 12 and 24hafter incubation in medium supplemented with glutamine.After12 hincubation,the proliferation effects in the groups supplemented with glutamine were significantly improved compared with the basal group,and no significant differences were observed in groups supplemented with glutamine,as well as the highest proliferation activity was found in 1mmol/L glutamine group.And the proliferation activity was decreased 24 hafter incubation except 2mmol/L glutamine group.The trend of killing ability against E.tardain the leukocytes was similar with the phagocytosis.The killing ability of leukocytes incubated in medium supplemented in 1and 2mmol/L glutamine were significantly higher than that in basal group（P〈0.05）,and no significant difference was observed in 0.5mmol/L glutamine group and the basal group（P〉0.05）.The findings indicate that glutamine is the effective immunomodulatory in grouper leukocytes,and the optimum glutamine supplemental level 1mmol/L at the incubation time of 12 h.