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P450CYP4F3表达对氢醌诱导MOLT4细胞凋亡的研究

Study of P450CYP4F3 expression on Apoptosis induced by hydroquinone in MOLT4 cells in vitro
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摘要 目的研究氢醌对体外培养条件下MOLT4细胞的毒性作用及细胞色素P450CYP4F3基因表达的意义。方法流式细胞术ANNEXINV-FITC加碘化丙啶(PI)双染定量检测细胞凋亡率和坏死率的变化;RT-PCR方法检测CYP4F3在mRNA表达水平,比较不同处理组之间的差异。结果加入不同浓度氢醌培养0、4、8、12、16 h后,流式细胞术检测结果发现,不同浓度氢醌作用后,细胞凋亡率明显高于空白对照组,差异有显著性(P<0.01),氢醌诱导细胞凋亡的最佳时间是8h,而随着作用时间延长,氢醌浓度为200μmol/L时,细胞坏死率明显增高;另外发现氢醌浓度为120μmol/L时,细胞凋亡率随着时间的延长逐步增加,8 h达到高峰,随后开始下降;CYP4F3基因在mRNA表达水平与细胞凋亡率一致。结论体外培养条件下,CYP4F3基因的表达上调可能对氢醌诱导MOLT4细胞凋亡起促进作用,并存在一定量-效与时-效关系。 Objective To study the cytotoxic effect induced by hydroquinone in MOLT4 cells in vitro,and the significance of cytochromo P450CYP4F3 expression.Methods Apoptotic and necrotic rate were examined by flow cytometer with Anti-AnnexinV /FITC Plus PI staining.The mRNA expression of CYP4F3 was detected by RT-PCR,and the differences in differently treated groups were compared.Results After adding different concentrations of hydroquinone to the cells for 0,4,8,12,16 h culture,the cell apoptotic rate in different concentrations of hydroquinone groups was significantly higher than that in blank control groups(P〈0.01).The optimal time induced by hydroquinone was 8 h.With time extension,the necrotic rate increased significantly at the concentration of 200 μmol /l.The apoptosis induced by hydroquinone was associated with culture time at the concentration of 120 μmol /l,and the peak apoptotic time was 8 h,then the apoptotic rate decreased.Furthermore,the amount of CYP4F3 expression at the mRNA level was in accordance with the apoptosis rate.Conclusions Hydroquinone could induce apoptosis of MOLT4 cells,the up-regulation of CYP4F3 expression probably promoted hydroquinone to induce MOLT4 cells to go into apoptosis in vitro with dose-effect and timeeffect relationship.
出处 《公共卫生与预防医学》 2014年第5期4-7,共4页 Journal of Public Health and Preventive Medicine
基金 国家自然科学基金资助(30170796)
关键词 氢醌 细胞色素P450 凋亡 坏死 Hydroquinone Cytochrome P450 Apoptosis Necrosis
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  • 1Sun R,Zhang J,Xiong M,e( al.Metabonomics biomarkers for sub-acute toxicity screening for benzene exposure in mice[ J] .J Toxi-col Environ Health A,2012,75( 18) :1163-1173.
  • 2Wang L,He X,Bi Y,ei al.Stem cell and benzene-induced malig-nancy and hematotoxicity[ J] .Chem Res Toxicol ,2012,25(7);1303-1315.
  • 3梁海荣,唐焕文,胡大林,庄志雄.小鼠吸入氢醌对肺外组织细胞DNA损伤的观察[J].广东医学院学报,2006,24(4):339-341. 被引量:1
  • 4Bi Y, Li Y, Kong M,et al. Gene expression in benzene-exposedworicers by microarray analysis of peripheral mononuclear bloodcells : induction and silencing of CYP4F3A and regulation ofDNA-dependent protein kinase catalytic subunit in DNA doublestrand break repair[ J] .Chem Biol Interact,2010,184(4) : 207-211.
  • 5Salmaan H, Inayat-Hussain, David Ross. Intrinsic pathway of hyd-roquione induced apoptosis occurs via both caspase-denpendentand caspase-independent mechanisms [ J ]. Chem Res Toxicol,2005,18(3):402-427.
  • 6徐持华,张国梁,赵职卫,夏颖,张秋萍,毕勇毅.用cDNA微阵列芯片检测苯中毒细胞色素P450基因表达差异[J].公共卫生与预防医学,2006,17(3):16-19. 被引量:2
  • 7Chen PH,Lee KW,Hsu CC,ei a/.Expression of a Splice Variantof CYP26B1 in Betel Quid-Related Oral Cancer [ J ]. ScientificWorld Journal,2014,7(8) :261-267.
  • 8Harris JB,Eldridge ML,Sayler G,et al.A computational approachpredicting CYP450 metabolism and estrogenic activity of an endo-crine disrupting compound( PCB-30) [ J] .Environ Toxicol Chem,2014,33(7):1615-1623.
  • 9Zhao Z,He X,Bi Y,al.Induction of CYP4F3 by benzene me-tabolites in human white blood cells in vivo in human promyelo-cytic leukemic cell lines and ex vivo in human blood neutrophils[J] .Drug Metab Dispos,2009,37(2) :282-291.
  • 10Christmas P,Tolentino K,Primo Y tet oi.Cytochrome P-450 4F18is the leukotriene B4 omega-1/omega-2 hydroxylase in mouse pol-ymorphonuclear leukocytes : identification as the functional orthologue of human polymorphonuclear leukocyte CYP4F3A in thedown-regulation of responses to LTB4[ J] .J Biol Chem,2006,281(11):7189-7196.

二级参考文献19

  • 1唐焕文,梁海荣,庄志雄,何云.低剂量氢醌诱导hPARP-1蛋白正常及缺陷细胞适应性反应研究[J].中华劳动卫生职业病杂志,2005,23(4):274-277. 被引量:4
  • 2唐焕文,梁海荣,庄志雄.低剂量氢醌诱导人胚肺成纤维细胞适应性反应研究[J].卫生研究,2005,34(5):529-532. 被引量:2
  • 3Aksoy M. Hematotoxieity and eareinogenieity of benzene. Environ[J]. Healtyment Perspect, 1989,82:193-197.
  • 4Snyder R, Kocsis J J. Crit Rev [J]. Toxicol, 1975,3: 265-288.
  • 5J萨姆布鲁克,E.F弗里奇,T曼尼阿蒂斯.《分子克隆实验指南》第二版,上海:科学出版社,1992.
  • 6Slobodan R, Frederick JC. Human cytochrome P450 enzymes:a status report summarizing their reactions, substrates, inducers, and inhibitors [J]. Drug Metabolism Reviews, 1997, 29(1&2) : 413-580.
  • 7Rothman N, Smith MT, Hayes RB, et al. Hematotoxieity among Chinese workers heavily exposed to benzene[J]. Am J Ind Med, 1996,29:236.
  • 8Yin S N, Rothma N, Dosemeci M, et al. A cohort study of cancer among benzene-exposed workers in China: Overall results[J]. Am J Ind Med, 1996,29:227.
  • 9Ioannides C, Parke DV. Induction of cytochrome P450A as indicate of potential chemical carcinogesis[J]. Drug Metab Rev,1993,25:485-501.
  • 10Kivistoe KT, Kroemer HK, Eichelbaum M. The role of human cytochrome P450 enzymes in the metabolism of anticancer agents: Implications for drug interactions[J]. Br J Clin Pharmacol, 1995,40:523-530.

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