不同精粗比日粮中添加甘露寡糖对绵羊体外消化率的影响

Efferts of adding different mannan-oligosaccharide to diets with different ratio of concentration to roughage on in vitro digestibility of sheep

为研究不同精粗比日粮中添加甘露寡糖(MOS)对体外干物质消化率(IVDMD)、有机物消化率(IVOMD)和粗蛋白质消化率(IVCPD)的影响,本试验以6只安装永久瘤胃瘘管羯羊(白萨福克♂×小尾寒羊♀)为瘤胃液供试动物,采用4×6二因子析因试验设计,共设2个处理,分别为不同精粗比[(A1(20∶80)、A2(30∶70)、A3(40∶60)、A4(50∶50)]和不同的甘露寡糖添加量[B1(0.0%)、B2(0.4%)、B3(0.8%)、B4(1.2%)、B5(1.6%)、B6(2.0%)]的日粮,进行两级离体消化试验,结果表明:A因子对日粮IVDMD、IVOMD和IVCPD均产生显著影响(P<0.05),均为A4处理组最高(63.98%、66.84%、28.05%),A1处理组最低(48.12%、51.11%、29.53%),B因子及A、B交互作用对日粮IVDMD、IVOMD和IVCPD无显著影响(P>0.05);多项指标综合指数(MFAEI)以40∶60日粮中添加1.6%MOS时为最大(0.6330),说明在40∶60日粮中MOS的添加水平为1.6%时能得到最佳组合效应。

The study was conducted to explore the effect of adding mannan-oligosaccharide （MOS） to diets with dif-ferent ratio of concentration to roughage on dry matter digestibility （IVDMD）,organic matter digestibility （IVOMD）and crude protein digestibility （IVCPD）in vitro of sheep by two-step technique.Six wethers （white Suffolk♂× Small-tailed Han sheep♀）installed permanent rumen fistula were used for offering rumen fluid.The 4 × 6 two-factor experimental de-sign was chosen and the two factors were the ratio of concentrate to roughage[A1（20∶80）,A2（30∶70）,A3（40∶60）,A4（50∶50）],and the dose of mannan-oligosaccharide[B1（0.0%）,B2（0.4%）,B3（0.8%）,B4（1.2%）,B5（1.6%）,B6 （2.0%）].The re-sults showed that the IVDMD,IVOMD and IVCPD were significantly influenced by the factor A （P〈0.05）,for the highest of group A4 （63.98%,66.84%,28.05%）,the lowest of group A1（48.12%,51.11%,29.53%）.The factor B and the interac-tion of both A and B didn’t significantly affect on IVDMD,IVOMD and IVCPD（P〈0.05）.Adding 1.6%MOS to 40∶60 di-et,the multiple-factors associative effects index was optimum （0.6330）,which showed that it could bring the optimum combined effect.