丹龙醒脑方促进大鼠神经干细胞增殖及其机制的研究

Effect of Danlong Xingnao Recipe on proliferation of neural stem cells in rats and its mechanism

目的探讨丹龙醒脑方对大鼠脑缺血再灌注后神经干细胞(NSC)增殖及神经生长因子(NGF)、碱性成纤维细胞生长因子(bFGF)和胶质源性神经营养因子(GDNF)表达的影响。方法 60只SD大鼠,随机分为假手术组、模型组、依达拉奉组(3.2mg/kg)、小剂量组(丹龙醒脑方3.7g/kg)、中剂量组(丹龙醒脑方7.48g/kg)、大剂量组(丹龙醒脑方14.8g/kg),每组10只。后5组大鼠大脑中动脉栓塞再通法建立脑缺血再灌注模型。各组大鼠治疗7d后,采用5-溴脱氧尿嘧啶核苷掺入法检测NSC增殖,用免疫组织化学法检测NGF、bFGF、GDNF表达。结果与假手术组比较,模型组NSC增殖数量显著升高、NGF、bFGF、GDNF表达均显著降低(P<0.05);与模型组比较,小、中、大剂量组NSC增殖数显著升高,中、大剂量组NGF、bFGF、GDNF表达均显著降低(P<0.05,P<0.01)。结论丹龙醒脑方可能通过促进NGF、bFGF、GDNF表达促进NSC增殖。

Objective To study the effect of Danlong Xingnao Recipe （DLXNR） on proliferation of neural stem cells （NSC） and expression of NGF ,bFGF ,GDNF after reperfusion in rats following cerebral ischemia .Methods Sixty SD rats were randomly divided into sham operation group , model group ,3 .2 mg/kg edaravone treatment group ,3 .7 mg/kg DLXNR treatment group ,7 .48 g/kg DLXNR treatment group and 14 .8 g/kg DLXNR treatment group （10 in each group） .A rat cerebral ischemia/reperfusion model was induced by middle cerebral artery occlusion and recanali-zation .Proliferation of NSC was detected by Brdu incorporating after the rats were treated for 7 days .Expressions of NGF ,bFGF and GDNF were detected by immunohistochemistry .Results The expression level of NGF ,bFGF and GDNF was significantly lower in model group than in sham operation group and in 7 .48 g/kg DLXNR treatment group and 14 .8 g/kg DLXNR treat-ment group than in model group （P〈 0 .05） .The number of proliferated NSC was significantly greater in 3 DLXNR treatment groups than in model group （P〈0 .05） .Conclusion DLXNR can stimulate the proliferation of NSC by up-regulating the expressin of NGF ,bFGF and GDNF .