γ-干扰素诱导蛋白-10及其受体CXCR3在结核性胸膜炎患者中的表达和意义

The expression of γ-interferon-inducible protein 10 and CXCR3 in tuberculous pleurisy

目的:探讨γ-干扰素诱导蛋白-10及其受体CXCR3在结核性胸膜炎患者外周血和胸水中的表达和临床意义。方法:采集30例健康正常者的外周血及30例结核性胸膜炎患者的外周血和胸水,采用流式细胞术检测结核性胸膜炎患者外周血单个核细胞及胸水细胞中的CXCR3的表达;用ELISA法检测结核性胸膜炎患者血清及胸水中IP-10的浓度。结果:结核性胸膜炎患者外周血血清中IP-10的浓度显著高于正常对照组(P<0.05),患者胸水中IP-10浓度较高,且患者胸水与患者外周血中IP-10水平具有正相关(r=0.572,P<0.05);结核性胸膜炎患者外周血中CXCR3的表达显著高于正常对照组(P<0.05),患者胸水细胞中CXCR3的表达较高,且患者胸水细胞与外周血中CXCR3表达具有正相关(r=0.627,P<0.05)结论:结核性胸膜炎患者血清中IP-10浓度及外周血单个核细胞CXCR3的表达显著升高,提示IP-10及其受体CXCR3是参与结核性胸膜炎发生发展的重要免疫分子,可能与结核性胸膜炎发病机制有关。

Objective： To investigate the expression of γ- interferon- inducible protein 10（ IP- 10）and its receptor CXCR3 in tuberculosis pleurisy patients,and explore their significance in tuberculosis pleurisy patients. Methods： Flow cytometry was used to detect the expression of CXCR3 in the peripheral Peripheral blood mononuclear cells and pleural fluid cells were isolated fromof tuberculosis pleurisy patients;. Flow cytometry was used to detect the expression of CXCR3. ELISA was used to detect the content of IP- 10 in serum and pleural fluid. Results： IP-10 in serum of tuberculosis pleurisy patients was significantly higher than that of the healthy normal controls（ P〈0. 05）,and IP- 10 in pleural fluid of tuberculosis pleurisy patients was elevated,both IP- 10 in serum and pleural fluid were positively correlated（ r = 0. 572,P〈0. 05）; CXCR3 in the peripheral blood mononuclear cell of tuberculosis pleurisy patients was significantly higher than that of the healthy normal controls（ P〈0. 05）,CXCR3 in pleural fluid cell of tuberculosis pleurisy patients was also elevated,both CXCR3 in the peripheral blood mononuclear cell and pleural fluid cell were positively correlated（ r = 0. 627,P〈0. 05）. Conclusion： The expression of IP- 10 and CXCR3 in tuberculosis pleurisy patient were significantly elevated,prompted suggesting that IP- 10 and CXCR3 may be the important molecules which may bethat related to the pathogenesis of tuberculosis pleurisy..