摘要
目的:探讨3种中药制剂肝复乐、黄芪注射液、β-榄香烯对Hep G2细胞增殖的抑制作用。方法:长满单层的对数生长期HepG2细胞,分别加入用DMEM完全培养基进行连续二倍稀释的中药复方肝复乐,以及黄芪注射液、β-榄香烯注射液,并设环磷酰胺药物对照与细胞空白对照,于37℃5%CO2分别培养48 h,采用MTT法测药物对Hep G2细胞的增殖、生长的抑制作用。结果:肝复乐、黄芪注射液、β-榄香烯对Hep G2细胞有明显的抑制作用,与空白对照组及环磷酰胺比较,差异均有统计学意义(P<0.05);3种中药对Hep G2细胞增殖的半数抑制率(IC50)分别为27.0 mg/mL、1.30 mg/mL、4.03 mg/mL。结论:肝复乐、黄芪注射液、β-榄香烯具有抑制Hep G2细胞增殖的作用,可为临床应用继续进行机理探讨。
Objective: To study the inhibitory effect on the proliferation of Hep G2 cells with GanFuLe, Astragalus injection and β-Elemene. Methods: Covered with a monolayer of Hep G2 cells in logarithmic growth phase were added to DMEM complete medium with continuous double dilution GanFuLe, Astragalus injection,β-Elemene injection, and set comparison among cyclophosphamide drug control and blank control, cells at 37℃5% CO2 were cultured 48 h, MTT assay was used to measure the drug Hep G2 ceils proliferation, inhibition of growth. Results: Compared with the blank control group and cyclophosphamide drug group, GanFuLe, Astragalus injection and β-Elemene produced significant inhibitory effects on Hep G2 cells(P〈0.05). The half inhibitory rate(IC50) of 3 kinds of Chinese herbal medicine on Hep G2 Cell proliferation respectively were 27.0 mg/mL, 1.30 mg/mL, 4.03 mg/mL.Conclusion: GanFuLe, Astragalus injection and β-Elemene all can inhibit the proliferation of Hep G2 ceils. And it worth continue to explore the mechanism.
出处
《中医药导报》
2014年第12期29-32,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy