奶牛场金黄色葡萄球菌的分离及耐药性研究

Study on Isolation and Drug Resistance of Staphylococcus aureus from Dairy Farm

为了解河南省规模化奶牛养殖场金黄色葡萄球菌的分布及耐药情况,从具有代表性的奶牛养殖场采集400批样品,采用VITEK-2 Compact全自动微生物分析系统对样品中的金黄色葡萄球菌进行分离鉴定,并通过微量肉汤稀释法对分离出的金黄色葡萄球菌进行耐药性分析。结果显示,该研究共分离金黄色葡萄球菌82株,分离率为20.5%。经耐药性分析发现,分离得到的金黄色葡萄球菌对青霉素、红霉素、氨苄西林耐药严重,耐药率分别为100%、86.6%和98.8%;对头孢菌素类、林可霉素类、喹诺酮类抗生素较为敏感。该研究结果提示河南省规模化奶牛养殖场金黄色葡萄球菌存在严重的耐药现象,建议在畜禽生产中要谨慎使用抗生素。

In order to understand distribution and drug resistance of Staphylococcus aureus of Henan Province. 400 samples were collected from dairy farms, and S. aureus were isolated and identified by VITEK-2 Compact automatic microbial analysis system,and the drug resistance analysis was carried out by mini-broth dilution susceptibility test. The result showed that total 82 isolates of S. aureus were isolated, and the isolating rate was 20.5%. The drug resistance analysis results showed that S. aureus had serious resistance to penicillin G, erythromycin and ampicillin, and the drug resistance rates were 100%, 86.6% and 98.8%. S. aureus was sensitive to cephalosporin, aminoglycoside and fluoroquinolone antibiotics.It is indicated that animal-origin S. aureus of Henan Province had serious resistance to antibiotics, so antibiotics should be cautious in animal production.