深圳市临床分枝杆菌菌种鉴定分析

Identification and analysis of mycobacterium isolated from clinical samples in ShenZhen

目的了解深圳地区分枝杆菌的感染、分布状况,为临床诊断和治疗提供科学依据。方法采用基因芯片,对1 096例临床疑似标本进行菌种鉴定。结果基因芯片鉴定结果阳性检出率为9.40%(103/1 096)。阳性患者中有87例为结核分枝杆菌感染,另有16例为非结核分枝杆菌感染,其中脓肿分枝杆菌5例、胞内分枝杆菌3例、鸟分枝杆菌3例、偶发分枝杆菌2例、堪萨斯分枝杆菌1例、海分枝杆菌1例、戈登分枝杆菌1例。结论深圳地区分枝杆菌感染,结核分枝杆菌占84.47%,为主要发病类型;非结核分枝杆菌分离率比例已达到15.53%,其感染菌株包括7个种类,因此开展分枝杆菌菌种鉴定,明确病因,对实现个体化治疗有着重要意义。

Objective To investigate the status and species of mycobacterial infections in Shenzhen for clinical diagnosis and treatment to provide a reliable scientific basis .Methods 1 096 of samples from patients with suspicious were detection by gene chip .Results Positive rate of microarray detection mycobacterium was 9 .40% （103/1 096） .103 cases of positive were 87 mycobac-teria by gene chip ,and 16 cases of non-tuberculosis（5 cases of M .abscessus ,3 cases of M .intracellulare ,3 cases of M .avium ,2 cases of M .fortuitum ,1 cases of M .kansas ,1 case of M .marinum ,1 case of M .gordonae .Conclusion The mycobacterial infections in Shenzhen ,tuberculosis infection as the main disease types （84 .47% ） .Non-tuberculous mycobacteria′s isolation ratio has reached 15 .53% ,including 7 kinds of species infection and one case of mixed infections .Identification of M ycobacterium by genechip have great significances for individualized treatment .