耐甲氧西林金黄色葡萄球菌耐药性及SCCmec基因分型研究

Antimicrobial resistance and SCCmec genotype of methicillin-resistant Staphylococcus aureus

目的了解大连地区耐甲氧西林金黄色葡萄球菌(MRSA)的耐药性及其葡萄球菌盒染色体SCCmec的基因型特点。方法采用纸片扩散法检测MRSA对抗菌药物的耐药性;聚合酶链反应(PCR)对SCCmec进行基因分型。结果分离出的38株MRSA菌株均具有多重耐药性,但对万古霉素和氯霉素较为敏感。SCCmec基因型以Ⅱ型最为常见,占86.84%;其次为Ⅲ型,占10.53%;未定型的占2.6%。结论大连地区分离出的MRSA具有多重耐药的特征,SCCmec基因型以Ⅱ型为主。

Objective To investigate antibiotic resistance and Staphylococcal cassette chromosome mec （SCC-mec） genotype of methicillin-resistant Staphylococcus aureus （MRSA）in Dalian Area .Methods Antimicrobial susceptibility of MRSA was detected by Kirby-Bauer method and SCCmec genotype by multiple PCR method .Results 38 strains of MRSA were all multidrug-resistant strains ,but susceptible to vancomycin and chloramphenicol .The most common SCCmec genotype was type Ⅱ （86 .84% ） ,type Ⅲwas in the second place（10 .53% ） ,and unidentified type accounted for 2 .6% .Conclusion MRSA strains in Dalian area are charac-terized by multidrug resistance ,type Ⅱ is the most common SCCmec genotype .