丹红注射液减轻DPPH所致离体心脏功能损伤的作用研究

Protective effects of Danhong injection on DPPH-induced injuries in the isolated hearts of rats

[目的]在大鼠离体心脏灌流系统上观察丹红注射液对二苯基三硝基苯肼(DPPH)自由基所致心功能损伤的保护作用。[方法]大鼠离体心脏采用Langendorff灌流,基础平衡30 min后进行各组预给药10 min后,用含0.25μmol/L DPPH的K-H液灌流心脏10 min,造成心脏功能损伤,最后用K-H液灌流10 min进行恢复。记录灌注压(PP)、左心室舒张压(LVEDP)、左心室收缩压(LVSP)、心率(HR)、左心室内压最大上升速率(+dp/dtmax)、左心室内压最大下降速率(-dp/dtmax),并计算心率压力乘积(RPP)。[结果]丹红注射液灌注10min可以显著降低灌注压,扩张冠状动脉。DPPH自由基损伤后RPP、+dp/dtmax显著降低,-dp/dtmax显著升高。用含丹红注射液的K-H液预处理10min,丹红2.5μL/mL组、丹红5μL/mL组可以减轻DPPH自由基导致的心脏功能损伤。[结论]在离体心脏模型中丹红注射液能扩张冠状动脉,减轻外源性自由基对心脏功能的损伤。

[Objective] To examine the protective effects of Danhong injection on DPPH-induced myocardial injuries in the isolated hearts of rats in Langendorff model. [Methods] The isolated hearts of rats were perfused in a Langendorff model for 30 min and pre-treatment with Danhong injection for 10 min. The hearts were subjected to Krebs-Henseleit（K-H） solution with DPPH 0.25 μmol/L for 10 rain to cause myocardial dysfunction, and followed by 10 rain recovery with K-H solution. The perfusion pressure （PP）, left ventricular systolic pressure （LVSP）, left ventricular end diastolic pressure （LVEDP）, heart rate （HR）, maximal and minimum rate of pressure development（± dp/dtmax） were recorded. And the heart rate pressure product （RPP） was calculated. [Results] The perfusion pressure significantly decreased after 10 min perfusion with Danhong injection. Perfusion with DPPH causes a remarkable decrease in RPP, ＋dp/dtmax and an increase in -dp/dtmax. Pretreatment with Danhong injection of 2.5 μL/mL and 5 μL/mL for 10 rain significantly reduced the DPPH-induced myocardial injuries. [Conclusion] Danhong injection can dilate coronary artery and protect against the myocardial injuries induced by DPPH in isolated rat hearts.