电针对缺血性中风后遗症期大鼠脑血流量和内皮素-1的影响

Effect of electroacupuncture on cerebral blood flow and secretion of ET-1 in rats with ischemic stroke sequelae

[目的]观察电针对缺血性中风后遗症期大鼠脑血流量的影响并探讨其作用机制。[方法]将30只Wistar成年健康雄性大鼠,按随机数字表法分为电针组、模型组、假手术组,每组10只。参照Zea-Longa线拴法复制大脑中动脉缺血模型(MCAO),MCAO 5周后为缺血性中风后遗症期大鼠模型。电针百会、水沟2周后,应用激光多普勒血流仪进行脑血流量的检测,免疫组化方法测定脑组织中内皮素-1(ET-1)含量。[结果]与假手术组比较,模型组脑血流量降低(P<0.05);与模型组比较,电针组脑血流量明显提高(P<0.05)。模型组ET-1蛋白表达阳性物质总面积高于假手术组(P<0.05)。电针组ET-1蛋白表达阳性物质总面积有低于模型组的趋势,但两组比较差异无统计学意义(P>0.05)。[结论]脑缺血后,脑组织中ET-1含量明显上升。电针有抑制ET-1上调的趋势,提高缺血性中风后遗症期大鼠的脑血流值。

[Objective] To observe the effect of electroacupuneture on cerebral blood flow and secretion of ET-1 in rats with isehemic stroke sequelae. [Methods] Thirty healthy adult male Wistar rats were randomly divided into electroaeupuncture group, model group and sham operation group with 10 rats in each group. Referencing the Zea- Longa line tied method the model of middle cerebral artery occlusion （MCAO） was reproduced. Five weeks later the model of ischemic stroke sequelae was considered to be formed. Rats in electroacupuncture groups were punctured on ＂Baihui＂, ＂Shuigou＂. Two weeks later, the Laser Doppler flow-meter was used to monitor the cerebral blood flow of rats and immunohistochemieal method was used for determination of the level of endothelin-1 （ET-1） in brain tissue. [Results] Compared with the sham operation group, the cerebral blood flow was reduced in the model group （P〈0.05）. Compared with model group, the cerebral blood flow was significantly increased in the EA group （P〈0.05）. In the model group the total area with ET-1 protein expression-positive substance was higher than that in sham group （P〈0.05）. The total area of the positive cells of ET-1 protein expression in the brain tissue of EA group had a lower trend than that in the model group. But there was no significant difference between these two groups （P〉0.05）. [Conclusion] After cerebral ischemia, cerebral tissue ET-1 levels has increased signifieantly. EA can inhibit the up- regulation of ET-1 and improve the blood flow of the rats with ischemic stroke sequelae.