多指标正交试验优选桂枝甘草汤提取工艺

Optimization of Extraction Process of Guizhi Gancao Tang by Multi-index Orthogonal Test

目的：优选桂枝甘草汤的提取工艺.方法：采用HPLC测定甘草苷、肉桂酸、桂皮醛及甘草酸含量,流动相乙腈（A）-0.1％磷酸水溶液（B）梯度洗脱（0 - 10 min,5％ - 20％A;10- 15 min,20％ - 23％A;15-30 min,23％ - 32％A;30 - 40min,32％ -35％ A;40 -50 min,35％ - 40％A;50 - 60 min,40％ - 50％ A）,检测波长254 nm.以甘草苷、肉桂酸、桂皮醛、甘草酸和浸出物提取量的综合评分为指标,采用正交试验考察加水量,提取次数,提取时间对提取工艺的影响.结果：桂枝甘草汤的最佳提取条件为加12倍量水回流提取3次,每次1h;甘草苷、肉桂酸、桂皮醛、甘草酸及浸出物提取量分别为5.93,11.73,12.25,12.34,172.86 mg·g-1.结论：多指标综合加权法能较为合理地反映桂枝甘草汤的整体药效,为该方的临床应用与开发提供实验依据.

Objective：To optimize extraction procedure of Guizhi Gancao Tang.Method：Contents of liquiritin,cinnamic acid,cinnamaldehyde and glycyrrhizic acid were determined by HPLC,mobile phase was consisted of acetonitrile （A）-water containing 0.1％ phosphoric acid （B） for gradient elution （0-10 min,5％-20％A; 10-15 min,20％-23％ A; 15-30 min,23％-32％ A; 30-40 min,32％-35％ A; 40-50 min,35％-40％ A; 50-60 min,40％-50％ A）,detection wavelength was at 254 nm.Orthogonal design was adopted to optimize extraction process with composite score of contents of liquiritin,cinnamic acid,cinnamaldehyde and glycyrrhizic acid as index,the amount of water,extracting time and times as factors.Result：Optimal extraction technology was：extracted thrice with 12 times the amount of water for 1.0 h each time; extracting amounts of liquiritin,cinnamic acid,cinnamaldehyde and glycyrrhizic acid were 5.93,11.73,12.25,12,34,172.86 mg ·g-1 Conclusion：Syhthetic weighted mark method can reasonably reflect overall efficacy of Guizhi Gancao Tang,this article provides experimental basis for clinical application and development of this compound.