HS-SPME-GC-MS测定艾叶挥发性成分方法优化

Methods Optimization About Analyze Volatile Constituents from Artemisiae Argyi Folium by HS-SPME-GC-MS

目的:建立顶空-固相微萃取-气相色谱-质谱(HS-SPME-GC-MS)联用分析艾叶挥发性成分的方法,研究不同因素对萃取效果的影响。方法:先做单因素试验,后采用L8(27)正交试验,以挥发性成分峰面积总和为指标考察最佳萃取条件,并考虑两两交互作用。结果:试验得出最佳萃取条件:样品1.0 g,80℃萃取40 min,100μm聚二甲基硅氧烷(PDMS)萃取头萃取,在250℃解吸附3 min检测,萃取手柄深度规固定在#2。用优化好的方法对艾叶样品进行分析,共分辨出84个色谱峰,准确鉴定出66个成分,占挥发性总成分的94.46%。结论:萃取温度为主要影响因素,其次是萃取温度与样品质量的交互作用,然后是萃取时间,最后是样品质量,除样品质量外其他因素都对萃取效果有显著性影响(P<0.05)。该方法为更好地开发利用艾叶提供依据。

Objective：To establish method of analyze volatile constituents from Artemisiae Argyi Folium by headspace solid-phase microextraction with gas chromatography-mass spectrometry （HS-SPME-GC-MS） technique,and analysis different factors＇ results on extraction effect.Method：After single factor test,the sum of the peak area of the volatile constituents was as index to optimum extraction conditions,while consider pair wise interactions.Result：The optimum HS-SPME parameters were selected as：1.0 g sample,80 ℃ extracted for 40 minutes.The volatiles were extracted using a 100 μm polydimethylsiloxane （PDMS） fiber,the desorption time was 3 minutes at 250 ℃ and adjustable depth gauge was #2.Analyzing the sample with the optimum methods,a total of 84 chromatographic peaks were resolved,and 66 constituents were identified,which accounted for 94.46％ of the total relative contents.Conclusion：Extraction temperature is the main factor,followed by the interaction between the extraction temperature and sample quality,then the extraction time,and finally the quality of the sample.Other factors have a significant impact on the extraction effect in addition to the sample quality （P ＜ 0.05）.It provides the basis for development and utilization of the Artemisiae Argyi Folium.