摘要
目的:提高榄葱茶的质量标准.方法:采用TLC对制剂中青果、紫苏叶、生姜进行定性鉴别,采用HPLC测定迷迭香酸和没食子酸的含量.结果:TLC鉴别斑点清晰,分离效果良好,阴性对照无干扰;迷迭香酸在0.098 -4.912 μg呈线性关系(r=0.999 9),平均回收率为100.24% (RSD 2.9%).没食子酸在0.1002-1.503 0μg呈线性关系(r=0.999 97),平均加样回收率为99.02% (RSD 1.8%).结论:所建立的方法专属性强、灵敏度高、重复性好,有利于该制剂的质量控制.
Objective:To improve the quality standard of Lancong Cha.Method:Canarii Fructus,Perillae Folium and Zingiberis Rhizoma Recens in the recipe were identified by TLC.The contents of rosmarinci acid and gallic acid were determined by HPLC.Result:The TLC spots were clearly and separated well,and the negative control did not impose any disturbance.Rosmarinci acid had good linearity in a range of 0.098-4.912 μg (r =0.999 9).The average recovery was 100.24%,and RSD was 2.9%.Gallic acid had good linearity in a range of 0.100 2-1.503 0μg (r =0.999 97).The average recovery was 99.02%,and RSD was 1.8%.Conclusion:The method was specific,sensitive and repeatable,can be used to control the quality of Lancong Tea better.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第21期75-78,共4页
Chinese Journal of Experimental Traditional Medical Formulae