基于UPLC/Q-TOF-MS的“小儿腹泻外敷散”敷脐治疗腹泻大鼠的尿液代谢组学

Metabonomic Study on Urine of Diarrheal Rats Treated with Xiaoer Fuxie Waifu Powder Based on UPLC /Q-TOF-MS

目的:研究小儿腹泻外敷散对番泻叶所致腹泻大鼠尿液代谢组学的影响,确定标志物,阐明治疗机制。方法:以超高压液相色谱-四级杆飞行时间串联质谱(UPLC/Q-TOF-MS)为核心技术,以主成分分析法(PCA)和偏最小二乘判别分析(PLS-DA)为数据解析手段,研究小儿腹泻外敷散对番泻叶所致腹泻大鼠的尿液中内源性代谢产物标志物的浓度影响。结果:各组之间样本点完全分离,各组内的样本有聚类作用,初步确定了6个生物标记物:马尿酸、柠檬酸、α-酮戊二酸、2-氨基苯甲酸、L-瓜氨酸、N-乙酰-5-羟色胺。小儿腹泻外敷散对6个生物标记物有明显的影响。结论:从生物体内代谢组学层面确定了小儿腹泻外敷散对番泻叶所致腹泻大鼠具有治疗作用,初步阐明了其治疗机制。

Objective：To investigate the effect of Xiaoer Fuxie Waifu powder on the urine of sennainduced diarrheal rats,identify the biomarkers,and explore its therapeutic mechanism.Method：Rat urine was collected and analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry （UPLC/Q-TOF-MS）.The acquired data were analyzed using principal component analysis （PCA） and partial least squares discriminant analysis （PLS-DA）.The concentration changes of endogenous metabolic biomarkers in the urine were studied.Result：Metabolite profiles of the urine samples among groups had been obviously classified.And samples showed clustering character in each group.Six metabolic biomarkers were tentatively identified as urobenzoic acid,citric acid,α-oxoglutarate,2-aminobenzoicacid,L-citrulline and N-acetyl-5-HTA,which could be obviously affected by Xiaoer Fuxie Waifu powder.Conclusion：The administration of Xiaoer Fuxie Waifu powder could affect endogenous metabolites of urine,which may be its therapeutic mechanism.