冠心病患者血清内脂素水平与炎症标志物及组织因子的关系

Relationship between serum visfatin and inflammatory markers and tissue factors in patients with coronary heart disease

目的探讨冠状动脉粥样硬化性心脏病(冠心病)患者血清内脂素与磷酸化c-jun、CD40L及组织因子(TF)的关系。方法选择不稳定型心绞痛患者65例(UAP组)、稳定型心绞痛(SAP)患者75例、对照组60例,采用ELISA方法测定血清内脂素、磷酸化c-jun、TF及组织因子途径抑制物(TFPI)浓度水平,采用流式细胞技术测定CD40L平均荧光强度。结果 UAP组患者血清内脂素、磷酸化c-jun、CD40L、TF、TFPI水平及TF/TFPI值高于SAP组及对照组(P<0.05或P<0.01);SAP组上述指标均高于对照组(P<0.05或P<0.01)。线性相关分析显示冠心病患者血清内脂素水平与磷酸化c-jun、CD40L、TF、TFPI水平及TF/TFPI值呈正相关(P均<0.01)。多重线性逐步回归分析表明冠心病患者血清内脂素水平与磷酸化c-jun、CD40L、TF水平呈独立正相关(P均<0.01)。结论血清内脂素水平升高可能促进炎症标志物磷酸化c-jun、CD40L及组织因子生成,影响冠心病发生、发展及预后。

Objective To investigate the relationship between serum visfatin and phosphorylated c-Jun,CD40L and tissue factors （TF）in patients with coronary heart disease.Methods In total,200 sub-jects were enrolled in this study and divided into the control group（n =60）,unstable（n =65）and stable angi-na groups（n =75 ）.Serum visfatin,phosphorylated c-Jun,TF and tissue factor pathway inhibitor （TFPI） were measured using ELLSA,while the percentage of positive CD40L was quantified by flow cytometry.Re-sults The serum levels of visfatin,phosphorylated c-Jun,CD40L,TF,TFPI and the ratio of TF /TFPI in the stable angina group were significantly higher compared with those in the control group（P 〈0.05 or P 〈0.01 ）.All these parameters in patients with unstable angina were significantly higher than their counterparts with stable angina （P 〈0.05 or P 〈0.01 ）.Linear correlation analysis revealed that serum visfatin was positively correla-ted with phosphorylated c-Jun,CD40L,TF,TFPI and TF /TFPI （all P 〈0.01 ）.Multiple linear stepwise re-gression analysis demonstrated that serum visfatin was independently and positively associated with phosphoryla-ted c-Jun,CD40L and TF（all P 〈0.01 ）.Conclusion Hypervisfatinemia probably influences the incidence, development and prognosis of coronary heart disease by elevating the levels of phosphorylated c-Jun,CD40L and TF.