固体三维荧光指纹图谱在三七品质鉴定中的应用

Identification of Panax notoginseng Base on Solid Three-Dimensional Fluorescence Fingerprint Spectra Technology

目的：建立三七和三七支根的固体三维荧光指纹图谱。方法：以血塞通冻干粉作为对照,采用固体三维荧光技术建立三七及三七支根的固体荧光指纹图谱。结果：血塞通冻干粉有230 nm/330 nm、280 nm/660 nm、300nm/340 nm、370 nm/590 nm、440 nm/550 nm、490 nm/610 nm的荧光峰,最强的为300 nm/340 nm的荧光峰。三七粉末具有230 nm/340 nm、290 nm/340 nm、370 nm/480 nm、430 nm/540 nm的荧光峰,最强为290 nm/340 nm的荧光峰;三七支根的荧光峰在280 nm/450 nm、290 nm/350 nm、350 nm/440 nm,最强为350 nm/440 nm的荧光峰。三者均有290~300 nm/340~350 nm的荧光峰,且血塞通冻干粉和三七均是该峰最强,而三七支根是350 nm/440 nm的荧光峰最强。三七和血塞通冻干粉还有230 nm/330~340 nm的荧光峰,三七支根没有。结论：通过该方法获得了相应样品的固体三维荧光光谱图。在建立的固体三维荧光光谱中选取以上两个荧光峰作为特征荧光峰进行检测,可以高效快速的鉴别三七和三七支根。

Objective：To establish the solid three-dimensional fluorescence fingerprint spectra for the main root and branch root of Panax notoginseng. Methods：With the Xuesaitong power as a control,the solid three-dimensional fluorescence technique was employed to establish the fingerprint spectra. Results：The fluorescence peaks of Xuesaitong power were at 230 nm /330 nm,280 nm /660 nm,300 nm /340 nm,370 nm /590 nm,440 nm /550 nm,and 490 nm /610 nm. Among of them,the 300 nm /340 nm peak was the strongest. The fluorescence peaks of Panax notoginseng main root power were at 230 nm /340 nm,290 nm /340 nm,370 nm /480 nm,and 430 nm /540 nm. Among of them,the peak at 290 nm /340 nm was the strongest. The branch root of Panax notoginseng was at 280 nm /450 nm,290 nm /350 nm and 350 nm /440 nm,and the peak at 350 nm /440 nm was the strongest. All of them had the peak at 290 ~ 300 nm /340 ~350 nm,this peak was the strongest in Xuesaitong and Panax notoginseng main root power,but 350 nm /440 nm was the strongest in the branch root of Panax notoginseng. Only the peak at 230 nm /330 ~ 340 nm was not detected in the branch root of Panax notoginseng. Conclusion：The solid three-dimensional fluorescence fingerprints spectra are established. Using peaks at 290 ~ 300 nm /340~ 350 nm and 230 nm /330 ~ 340 nm as characteristics of the fluorescence peak can efficiently identify main root and branch root of Panax notoginseng.