夏桑菊颗粒UPLC指纹图谱研究

Study on UPLC Fingerprint of Xiasangju Granules

目的:建立不同批次夏桑菊颗粒的指纹图谱。方法:采用UPLC,建立12批夏桑菊颗粒的指纹图谱。ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm),流动相为乙腈-0.5%醋酸溶液,梯度洗脱,柱温30℃,流速0.4 mL/min,检测波长320 nm,标定了16个色谱峰,分别采用相似度软件、聚类分析和主成分分析等方法,对12批样品进行系统的比较与归类。结果:建立了夏桑菊颗粒专属性的UPLC指纹图谱,将不同批次的样品分为6大类。结论:该方法重复性好,简便可靠,可用于夏桑菊颗粒的快速鉴别,可为夏桑菊颗粒的质量控制提供依据。

Objective：To establish a UPLC fingerprint method of Xiasangju Granules. Methods：UPLC analysis was performed on a Waters ACQUITY UPLC H-Class system and carried out at 30 ℃ on a Waters Column ACQUITY UPLC BEH C18（2.1 mm ×50 mm,1. 7 μm）. A binary gradient elution system was composed of acetonitrile（phase A）and 0. 5% acetic acid solution（phase B）. Detection was performed at the wavelength of 320 nm,the mobile flow rate was at 0. 4 mL /min. A matrix including 16 variations（ characteristic peaks area）and 12 samples was constructed for similarity evaluation,cluster analysis and principle component analysis. Results：The results showed that the collected samples had a good similarity. A specificity fingerprint was produced and 16 characteristic peaks were designated. 12 samples were divided into 6 groups. Conclusion：It is a reliable,available and quick method for quality control of Xiasangju Granules.