植物△1-吡咯啉-5-羧酸合成酶（P5CS）基因的研究进展

Advance in △1-pyrroline-5-carboxylate synthase （P5CS） gene in plants

△1-吡咯啉-5-羧酸合成酶(P5CS)是植物脯氨酸生物合成的关键酶。近年来,许多学者对P5CS酶基因克隆、其在植物中的表达特性与在抗逆基因工程上的应用进行了大量研究,证实P5CS基因已成功在许多植物中进行过量表达并能有效提高植物的抗旱、耐盐性。随着分子生物学和现代生物技术的快速发展,今后可进一步挖掘利用植物P5CS基因,有效验证其功能和了解其作用机制,探讨P5CS基因与其他基因的互作及调控植物抗逆的生理生化机理,研究其在植物生长发育及器官发生等过程中的作用;培育出高效、实用、抗逆性强的转基因植物品种,均有待今后进行深入研究。

△1-pyrroline-5-carboxylate synthase （P5CS） is a key enzyme in plant proline biosynthesis. In recent years, P5CS gene cloning and its expression characteristic and application on stress resistance genetic engineering were studied widely. PSCS gene has been proved to be successfully overexpressed in many plants and could improve plant drought resistance and salt-tolerance. With the rapid development of molecular biology and modern biotechnology, P5CS gene in more plants should be studied to verify its function and role mechanism. The interaction of P5CS gene with other genes and their physiological-chemical mechanism on regulating plant resistant stress should be explored. The role of P5CS gene on growth and develcpment, and organogenesis of plant should be further studied. Further- more, transgenic plant varieties with high-efficiency, practical and strong stress resistance should be bred to improve plants resistant to adversity stress in the future.