摘要
以活性污泥为原料,采用单因素试验法,分别对影响活性污泥中蛋白质提取效率的7个因素进行了研究。结果表明:当Na Cl溶液浓度为2%、抽提p H值为8.5、抽提温度为25℃、抽提时间为2 h、沉淀温度为5℃、沉淀p H值为3、样品∶抽提液=1∶20、沉淀时间为16 h时,蛋白质的提取率最高,为17%左右。
Seven factors influencing the extraction efficien tivated sludge were investigated respectively using the single cy of factor protein from microorganisms in ac- experiment method. The results showed that the maximum protein extraction rate was about 17% at NaCI solution concentration of 2%, extraction pH of 8.5, extraction temperature of 25 ℃, extraction time of 2 h, precipitation temperature of 5 ℃, precipitation pH of 3, sample ： extract of 1 ： 20 and precipitation time of 16 h.
出处
《中国给水排水》
CAS
CSCD
北大核心
2014年第21期124-127,共4页
China Water & Wastewater
关键词
活性污泥
蛋白质
提取
单因素试验
activated sludge
protein
extraction
single factor experiment method
