摘要
目的利用Maxwell 16裂解纯化法从保存8年以上陈旧精斑检材中获取精子DNA。方法 8份陈旧精斑检材采用Maxwell 16裂解纯化法提取精子DNA,并采用Powerplex○R21试剂盒进行复合扩增,产物用AB3130型遗传分析仪检测,结果与常规方法进行对比。结果成功获得8份陈旧精斑检材精子STR分型。结论差异裂解配合Maxwell 16裂解在陈旧精斑检材精子DNA检验中效果明显。
In this paper, DNA from 10 years old semen samples was extracted by using Maxwell 16 and the results were compared with conventional extraction methods. The study indicated that Maxwell 16 is effective for extracting DNA from old se men samples,
出处
《刑事技术》
2014年第5期12-13,共2页
Forensic Science and Technology
