深圳地区9569例妊娠期妇女血红蛋白电泳结果分析

Analysis on the hemoglobin electrophoresis results of 9 569 pregnant women in Shenzhen City

目的评估全自动毛细管血红蛋白(Hb)电泳技术在深圳地区妊娠妇女产前珠蛋白生成障碍性贫血(地贫)筛查及异常Hb检查的临床应用价值。方法采用Sebia capillary2flex型全自动毛细管电泳仪对深圳市龙岗区妇幼保健院产前门诊孕妇进行Hb电泳检测,分析HbA2水平及异常Hb电泳条带及水平。结果检出951例异常Hb电泳结果(异常率9.9%)包括HbA2>3.5%;HbA2<2.5%;HbF>10%及其他异常Hb(包括HbCS、HbE、HbD、Hb NewYork和Hb Q-Tailand等)。结论全自动毛细管Hb电泳在地贫产前筛查及异常Hb检测中具有重要意义,可为进一步分子确诊及产前诊断提供指导。

Objective To evaluate the clinical value of the automatic capillary hemoglobin electrophoresis tech -nology in both the screening of prenatal thalassaemia and the detection of abnormal hemoglobin in pregnant women in Shenzhen .Methods The Sebia capillary 2-type automatic capillary electrophoresis apparatus was employed to detect the hemoglobin electrophoresis spectrum of the 9 569 prenatal outpatients .The content of hemoglobin A2 ,the bands and contents of other abnormal hemoglobins were analyzed .Results Abnormal hemoglobin electrophoresis spectrum was detected in 951 prenatal outpatients （with an abnormal detection rate of 9 .9% ） ,including hemoglobin A2 〉3 .5% ,hemoglobin A2 〈 2 .5% ,hemoglobin F 〉 10% and other abnormal hemoglobins（including hemoglobin CS ,he-moglobin D ,hemoglobin E ,hemoglobin NewYork and hemoglobin Q-Tailand ,etc） .Conclusion Automatic capillary hemoglobin electrophoresis is of great significance in the screening of prenatal thalassaemia and the detection of ab -normal hemoglobin in pregnant women ,which could provide guidance for further definite molecular diagnosis and pre-natal diagnosis .