老年患者感染黏液型铜绿假单胞菌的药敏结果分析

Analysis on susceptibility results of mucoid Pseudomonas aeruginosa in elderly patients

目的分析老年感染黏液型铜绿假单胞菌的药敏结果和抗菌药物的灵敏性,为临床抗菌药物的合理应用提供理论依据。方法提取66株老年感染黏液型铜绿假单胞菌进行体外培养,并分别对菌株进行传代培养。同时对菌株在抗菌药物中不同培养时间点的最低抑菌浓度和抑菌环大小进行测定,检测不同抗菌药物对黏液型铜绿假单胞菌耐药性。然后对数代传种后黏液消除的菌株进行相同检测,对比两次检测结果并进行分析。结果传种前抑菌环直径在24h与48、72h及传种后24、48、72h的检测结果比较,差异具有统计学意义(P<0.05)。其中48、72h检测结果与传种后24、48、72h的测量结果比较,差异无统计学意义(P>0.05)。广泛的抗菌药物对铜绿假单胞菌灵敏性都比较高,但对黏膜性菌株耐药性明显增强,需要考虑生物膜的耐药作用。结论 48h内检测老年感染黏液型铜绿假单胞菌有重要的诊断和疗效意义。对黏液型铜绿假单胞菌需要考虑生物膜的耐药作用,尽量提高抗菌药物使用的合理性。

Objective To analyze the susceptibility results of mucoid Pseudomonas aeruginosa in elderly pa -tients ,provide basis for the rational use of clinical antibacterial drugs .Methods A total of 66 strains mucoid Pseudo-monas aeruginosawere in elderly patients were extracted and cultured in vitro ,and then conducted subculture .Detec-ted the minimum inhibitory concentration （MIC） and bacterial inhibition ring at different culture period in different antibiotics .Then after passing several generations ,the same kinds of tests were made to detect eliminated mucus strains .Results were compared between the two tests .Results Bacterial inhibition ring after culturing 24 hours ,48 hours ,72 hours had significant differences（P 〈 0 .05） .Results after culturing 48 hours ,72 hours had no significant differences compared with the results at 24 hours ,48 hours ,72 hours after subculture （P 〉 0 .05） .Conclusion It is value to detect mucoid Pseudomonas aeruginosa in elderly patients in 48 hours after culture .