中药复方制剂治疗急性感染弓形虫小鼠的效果研究

Efficacy of a traditional Chinese medicine compound preparation against artificial infection of Toxoplasma gondii NT strain in mice

观察研制的中药复方制剂治疗急性感染弓形虫小鼠的效果。在测定感染弓形虫小鼠最小致死量的基础上,比较不同剂量的中药复方制剂(35、25和15 g/kg)和磺胺间甲氧嘧啶钠(300、200和100 mg/kg)的疗效。为进一步验证治疗剂量的效果,每只鼠经腹腔感染25个弓形虫速殖子后4 h,鼠逐只经胃灌服25 g/kg中药复方制剂或200 mg/kg磺胺间甲氧嘧啶钠,每日2次,连用5 d,同时设感染不用药组与空白对照组,以小鼠存活率与平均存活时间、腹腔液中虫体数、组织病变程度以及SOD活性等指标评价药物效果。不同剂量治疗效果的比较显示,中药高剂量组、中剂量组与磺胺间甲氧嘧啶钠中剂量组的小鼠存活率、平均存活时间及腹腔液中虫体数相近,三者间差异不显著(P>0.05)。验证试验显示,中药复方制剂组(25 g/kg)与磺胺间甲氧嘧啶钠组(200 mg/kg)的小鼠存活率、平均存活时间、腹腔液中虫体数和血清SOD活性相近,两者间无显著差异(P>0.05),两者均能减轻肝、肺、脾的组织病变。中药复方制剂具有一定的抗弓形虫效果,25 g/kg的剂量效果最佳。

The efficacy of a traditional Chinese medicine compound preparation against acute murine toxoplasmosis was evaluated in this study. The minimum lethal dose in mice infected by Toxoplasma gondii was determined. The efficacies of traditional Chinese medicine com- pound preparation at different doses （35 g/kg, 25 g/kg, 15 g/kg） and different doses （300 mg/kg, 200 mg/kg, 100 mg/kg） of sulfamon- omethoxine sodium were compared to screen optimal drug dose. Then the efficacies at optimal drug dose were further confirmed as the follow- ing procedures. Each mouse except those in negative control was intraperitoneally inoculated with 25 tachyzoites of T. gondii NT strain. Four hours later, each mouse except those in negative control group and challenged untreated group were treated with traditional Chinese medicine compound preparation （25 g/kg ） or sulfamonomethoxine sodium （200 mg/kg ） by intragastric administration, twice per day for 5 days, re- spectively. The efficacies of drugs were assessed by survival rates and mean survival days of mouse, numbers of tachyzoite in peritoneal exu- dates, degree of pathological changes of major tissues and activities of SOD in serum. The results showed that there were no significant differ- ences （P〉0.05） in survival rates and mean survival days of mouse, and numbers of tachyzoite in peritoneal exudates among the three groups, which were treated with 35 g/kg or 25 g/kg traditional Chinese medicine compound preparation and 200 mg/kg sulfamonomethoxine sodium, respectively. The further test showed that the results of above indexes were similar between 25g/kg of traditional Chinese medicine compound preparation and 200 mg/kg of sulfamonomethoxine sodium, and both treatments could lighten the degree of pathological changes of liver, lung and spleen. It is concluded that traditional Chinese medicine compound preparation had a certain effect against T. gondii, and the efficacy was best at the dose of 25 g/kg.