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鸡刺鼠相关蛋白多肽抗体的制备与鉴定

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摘要 制备鸡刺鼠相关蛋白(agouti-related protein,AgRP)多肽抗体并鉴定。根据GenBank中报道的AgRP一级结构信息,用TMHMM 2.0和DNA Star软件分析其蛋白跨膜结构域、二级结构、疏水性、亲水性等,设计出1段13个氨基酸的多肽。将合成后的多肽与牛血清白蛋白(BSA)偶联,用与BSA偶联的AgRP多肽免疫新西兰大白兔,获取血清后亲和纯化出抗AgRP多肽抗体。通过ELISA法检测效价,Western blot和免疫组化法检测抗体特异性。结果获得了高效价的鸡AgRP多肽抗体,ELISA法测定其效价可达到1∶128 000,并可用于免疫组化研究。
作者 毕雪 郎斌 王佳美 朱弘焱 解放 苏玉虹 田玉民
机构地区 辽宁医学院畜牧兽医学院
出处 《畜牧与兽医》 北大核心 2014年第10期74-77,共4页 Animal Husbandry & Veterinary Medicine
基金 国家自然科学基金(31172242) 辽宁医学院校长基金-奥鸿博泽研究生科研创新基金
关键词 鸡 刺鼠相关蛋白(AgRP) 多肽抗体
分类号 S852 [农业科学—基础兽医学]
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参考文献10

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畜牧与兽医
2014年 第10期

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