GC-MS分析南葶苈子挥发油成分的研究

Study on Essential Oil in the Seeds of Descurainia Sophia(L.) Webb ex Prantl by GC-MS Analysis

目的:首次运用GC-MS结合保留指数分析南葶苈子挥发油成分,为进一步研究南葶苈子挥发油提供实验依据。方法:采用水蒸气蒸馏法提取挥发油,用气相色谱-质谱联用(GC-MS)技术并结合保留指数对其进行分析鉴定。结果:采用GC-MS分析检测出33个组分,利用MS结合保留指数定性,鉴定出其中28个成分,占总检出化合物的99.91%。其中含量较高的组分为3-亚甲基-壬烷,占68.14%,其次为嘧啶(29.32%)、2-庚烯醛(0.58%)、哌啶-3-甲酸甲酯(0.43%)、4-氧代丁腈(0.31%)、8-氯-新异长叶烯(0.25%)等。结论:该方法可以提高定性结果的准确性。

The analysis of essential oil in seeds ofDeseurainia sophia provided an experimental basis for further research on essential oil activity test at the first time. Essential oil was extracted by steam distillation method. Analysis and identification were made by gas chromatography-mass spectrometry （GC-MS） technology in combination with retention indices. A total of 33 components in seeds ofD. sophia were detected by GC-MS and 28 compounds were identified by MS in combination with Kovats retention index. The compounds with high contents were as follows： 3-methylene-nonane （68.14%）, 1,3-diazine （29.32%）, 2-n-butylacrolein （0.58%）, methyl nipecotate （0.43%）, 4-oxo-butanenitrile （0.31%）, 8-chloro-neoisol-ongifolene （0.25%） and so on. It was concluded that 28 volatile components were identified by GC-MS combined with retention indices. The total detected components were 99.91%. This method was able to improve the accuracy of qualitative detection results.