西瓜藤石油醚提取物抗炎作用及其机制探讨

Study on Anti-inflammatory Effects and Mechanism of Petroleum Ether Extract from Citrullus lanatus Vine

目的:研究西瓜藤石油醚提取物的抗炎作用并探讨其作用机制。方法:采用二甲苯致小鼠耳肿胀模型、小鼠腹腔毛细血管通透性增高模型、鸡蛋清致小鼠足肿胀模型、棉球致大鼠肉芽肿模型、角叉菜胶致大鼠足肿胀模型,观察西瓜藤石油醚提取物的抗炎作用。并测定棉球肉芽肿模型大鼠血清中一氧化氮(NO)、一氧化氮合酶(NOS)的含量和肿瘤坏死因子-琢(TNF-琢)、白细胞介素-1茁(IL-1茁)水平;测定角叉菜胶足肿胀模型大鼠肿足炎性渗出物中丙二醛(MDA)、前列腺素E2(PGE2)、5-羟色胺(5-HT)、组织胺(His)、蛋白含量及血清中NO、NOS的含量及总超氧化物歧化酶(SOD)活性,探讨可能的抗炎机制。结果:西瓜藤石油醚提取物对二甲苯致小鼠耳肿胀、小鼠腹腔毛细血管通透性、蛋清致小鼠足肿胀、棉球致大鼠肉芽肿、对角叉菜胶致大鼠足肿胀,均有不同程度的抑制作用。能降低棉球肉芽肿大鼠血清中NO、NOS含量和TNF-琢、IL-1茁水平,能降低角叉菜胶足肿大鼠肿足炎性渗出物中MDA、PGE2、5-HT、His及蛋白含量,抑制血清中NO和NOS的升高,提高SOD的活性。结论:西瓜藤石油醚提取物具有一定的抗炎作用,其机制可能与影响炎症介质的产生和抗氧化作用有关。

This article was aimed to study anti-inflammatory effects of petroleum ether extract from Citrullus lanatus vine （PEECLV） and explore the mechanism. The methods of ear swelling with xylene, increasing of peritoneal capil-lary permeability, and paw edema with egg albumin were used in the establishment of mice models, respectively. Granuloma with cotton pellet, and paw edema with carrageenan were used in the establishment of rat models, re-spectively. Observation was made on anti-inflammatory effects of PEECLV. The content of nitrous oxide （NO） and nitric oxide synthase （NOS）, as well as the level of TNF-α and IL-1β in blood serum were measured among granu-loma with cotton pellet rat models. Contents of malondialdehyde （MDA）, prostaglandin E2 （PGE2）, 5-HT, histamine （His）, and protein in inflammatory exudates from the paw edema with carrageenan rat model, as well as contents of NO and NOS in blood serum, and activity of superoxide dismutase （SOD） were measured in order to study the possi-ble anti-inflammatory effects. The results showed that PEECLV can suppress the ear swelling with xylene, the in-creasing of peritoneal capillary permeability, and the paw edema with egg albumin in mice, as well as granuloma with cotton pellet, paw edema with carrageenan in rats. It had anti-inflammatory effects at different degrees. The content of NO and NOS, as well as the level of TNF-α and IL-1β in blood serum were reduced in granuloma with cotton pellet rat models. The content of MDA, PGE2, 5-HT, His, and protein content were reduced in inflammatory exudates from the paw edema with carrageenan rat model. The increasing of NO and NOS in blood serum was inhib-ited. And the activity of SOD was increased. It was concluded that PEECLV had certain anti-inflammatory effects. Its mechanism may be related to the influence of the production of inflammatory mediators and antioxidant.