BHK21细胞的悬浮驯化及其悬浮培养参数研究

Study on BHK21 Cell Suspension Adaptation and Culture Parameters in Suspension Condition

[目的]探讨各种悬浮培养条件对BHK-21细胞悬浮培养的影响,尤其是细胞生长过程中出现的细胞结团现象对细胞生长状态的影响,从而摸索出稳定的培养条件。[方法]将BHK-21细胞株进行悬浮驯化培养,通过对悬浮培养的培养液、pH、溶解氧、罐压、搅拌等各种细胞相关生长条件进行研究。[结果]细胞的结团严重影响了细胞的生长代谢。最佳培养条件为最佳接种浓度0.2×106个/ml,牛血清的最佳含量为5%,BHK21细胞培养液理想pH为7.0~7.2,采用2孔通气的100 r/min有利于BHK21细胞的生长。[结论]为体外规模化细胞培养平台奠定理论基础。

[Objective] The aim was to discuss the effect of various cultivate condition on BHK-21 cell suspension cultures,especially the effect of cell aggregation phenomenon of cell growth in the process of the cells growth state, and the stable culture condition were found out. [ Method ] The BHK-21 cells were suspended domestication,cultivation of suspension culture by nutritive medium, pH, dissolved oxygen, tank pressure for mixing all kinds of cell growth conditions were studied. [ Result] Cluster ceils greatly influenced the growth of the cell metabolism. The best culture conditions were optimal inoculation concentration of 0.2 x 106/ml ,the best content of bovine serum 5% ,the ideal BHK21 cell cultures of pH 7.0 - 7.2 ,the two ventilation hole 100 r/min speed of BHK21 cell growth. [ Conclusion] The study laid a theoretical foundation for large-scale cell culture in vitro platforms.