不同基因型亚麻花粉小孢子离体培养研究

Study on Micro Propagation of Pollen Microspores of Different Genotypes Flax in vitro

[目的]研究不同基因型亚麻花粉小孢子离体培养,建立亚麻花粉小孢子离体培养再生体系,以期加快亚麻单倍体育种进程。[方法]以5种不同基因型亚麻的花粉小孢子为外植体,研究不同基因型亚麻愈伤组织诱导率、绿苗的分化率的差异,筛选适宜亚麻花粉小孢子培养基。[结果]不同基因型的亚麻花粉愈伤组织的诱导率、绿苗分化率差异显著,9601和9605愈伤组织的诱导率较高,分别为30.8%和28.6%;9601组合的花粉绿苗分化频率最高为14.5%。其次为9718和9712,分化率分别为11.5%和8.0%;筛选出亚麻花粉小孢子适宜的愈伤组织诱导培养基HF1、绿苗分化培养基HF2。[结论]初步建立了亚麻花粉小孢子离体培养再生体系,为亚麻单倍体育种、突变体筛选、转基因等研究奠定了基础。

[ Objective ] The aim was to accelerate breeding course of flax haploid by studying the micro propagation of pollen microspores in vitro and establishing regeneration system of different flax genotypes. [ Method] In vitro culture of pollen microspores of five different flax genotypes were conducted by pollen microspores to explore the callus induction rate, seeding regeneration rate and select suitable medium of pollen microspores. [ Result ] The result showed that there were significant differences in callus induction rate and seeding regeneration rate. Callus induction rates of 9601 and 9605 were higher, 30.8% and 28.6% respectively. The highest seeding regeneration rate of 9601 was 14.5%, followed by 9718 and 9712, 11.5% and 8.0% respectively. HF1 that was suitable inductive medium of callus and HF2 that was medium of seeding regeneration were selected. [ Conclusion] The regeneration system of flax pollen microspores was established preliminarily. It provided foundation for haploid breeding, mutant screening and transgene of flax.